文档介绍：一种基于葡萄糖氧化酶的新颖葡萄糖电化学传感器的研究中文摘要
一种基于葡萄糖氧化酶的新颖葡萄糖电化学传感器的研究中文摘要
葡萄糖氧化酶是一种重要的生物大分子,是一种葡萄糖的高效氧化催化酶,研究葡萄糖氧化酶的电化学现象在生命科学中具有重要的意义。本论文集自组装、纳米技术和溶胶一凝胶技术于一体,一步法同时有序地固定葡萄糖氧化酶和金溶胶于三维硅溶胶一凝胶网络结构中,实现了葡萄糖氧化酶在金电极表面上有效和稳定的直接电子转移。探讨了酶固定量、L-半胱***酸量对循环伏安极峰电流的影响,并优化了电极制备的实验条件。~+,阴阳峰电位之差为AED=210 mV(扫速为100 mV s。1),根据(Ep。+Epc)/2估算可得到式量电位为110 mV(), GOD/Cys/AuNP/ s~。进一步的实验结果显示,固定在GOD/Cys/AuNP/,而且电催化活性很稳定,酶电极对葡萄糖检测的响应时间小于5 s,线性范围是10—1301amol L-1,检测限为3pmol (信噪比3),按照 Lineweaver-Burk方程得到GOD/AuNP/Cys/sol—gel/Au电极的Michaelis—Menten常数(1(M8■ mmol L-1,GOD/AuNP/Cys/sol—gel/Au电极在4"C下放置100天后对葡萄糖的响应仍保持原来的92%。实验结果表明:GOD/AuNP/Cys/,明显缩短了传感器的响应时间,降低了传感器的工作电压,并具有抗干扰的特点。该方法制备电极简单易行、易于操作, 并可用于其他生物氧化还原蛋白质和酶的固定。
关键词:葡萄糖氧化酶、电化学传感器、直接电子转移
作者:高盐生指导老师:狄俊伟
一种基于葡萄糖氧化酶的新颖葡萄糖电化学传感器的研究英文摘要
Abstract
Be of an important enzyme,glucose oxidase(GOD)has good electrocatalytic ability and hi曲selectivity tO research of electrochemistry of GOD has a vital significance in life sciences。bining self-assembled monolayers(SAMs), nanotechnology and sol—gel technology,a novel GOD biosensor was developed using a one—step method which simultaneously immobilized GOD and gold nanoparticles in three silica sol- on the surface of prepared gold electrodes。The preparation conditions were biosensor was prepared under the optimized conditions and the direct electron transfer between the active center of enzyme and electrode surface was pair of well—defined redox peak was observed at the enzyme electrode in
cyclic current ration of the cathodic peak current tO the anodic one is
nearly 1 and the separation between the anodic and cathodic peak potentials (△Ep=Ep8一Epc; V at lOOmV s-1)indicate that the electrode process of GOD is quasi— formal potential Eo。,calculated according to average of anodic and
cathodic peak pot