文档介绍：ABSTRACT
Objective:
Through intraperitoneal injection of streptozocin to establishe diabetic rats model, and observe the effect of dexmedetomidine(DEX) on humoral immune funct
-ion ponent function, to investigate the role of dexmedetomidine on humoral immune function in diabetic rats.
Method:
Seventy-two male Sprague-Dawley rats, weighing 300-350 g ,were randomly divided into 3 groups (n=24 each): control group (group C), diabetes mellitus group(group DM) and DEX + diabetes mellitus group(group DEX). The model of diabetes in group DM and group DEX was induced by intraperitioneal streptozocin 60mg/kg and confirmed by blood glucose ≥,and underwent 30 min of exploratory laparotomy starting from 4 weeks after the diabetes model. Dexmedetom
-idine 1μg/kg was infused iv over 10 min before operation, and then was infused at a rate of 5μg/kg·h maintain 4h in group DEX. Group C and group DM received the equal volume of normal saline. Six animals from each group selected before
operation(T0), at the end of operation, 24 and 72h after operation(T1 ~T3), the blood
glucose was recorded, blood samples were obtained for determination of serum concentration of IgA,IgG,IgM,CH50,C3 and C4. The body weight and weight of spleen were measure for spleen index.
Result:
Compared with group C, the blood glucose concentration was increased at T0 ~ 3 in group DM and group DEX(P<). Compared with group DM, the blood glucose concentration was decreased at T1~3 in group DEX(P<),and there was no significant difference at T0.
Compared with group C, the serum concentration of IgG and IgM were decreased, the serum concentration of IgA was increased at T0 ~ 3 in group DM and group DEX(P<). Compared with group DM, the serum concentration of IgG and IgM were increased, the serum concentration of IgA was decreased at T1 ~ 3 in group
DEX(P<).
Compared with group C, the serum concentration of CH50,C3 and C4 was increased at T0 ~ 3 in group DM and group DEX(P<). Co