文档介绍：第三军医大学
硕士学位论文
ATRA增强HSV-TK/GCV对膀胱癌旁观者效应的实验研究
姓名:罗顺文
申请学位级别:硕士
专业:外科学(泌尿外)
指导教师:叶钢
20040501
第三军医大学硕士学位论文
英文缩写一览表

英文缩写英文全称中文全称
ATRA All trans retinoic acid 全反式维甲酸
DMSO Dimethyl sulfoxide 二甲基亚砜
DEPC Diethyl pyrocarbonate 焦碳酸二乙酯
5’-LTR 5’-long terminal repeat 5’长末端序列
EDTA Ethylendiaminotetracetic acid 乙二安四乙酸
PBS Phosphate buffer saline 磷酸缓冲生理盐水
LB Luria bertani LB 培养基
TE Tris –Hcl EDTA TE 缓冲液
RT-PCR Revert-transcription poly-chain reaction 反转录 PCR
P-gp P-glycoprotein 糖蛋白
KD Kilo dulun 千道尔顿
Escgerichia Coli 大肠杆菌
DOTAP N,N,N,-trimethylammonium methyl sulfate 脂质体
EB Ethidium bromide 溴化乙锭
Opical density 光密度
H .B Hygromycin B 潮霉素 B
d NTP Deoxybonucleotide triphosphate 寡脱氧三磷酸苷
NBT Nitrio blue tetrazoliu chloride 硝基四氮唑蓝
DAB diaminobezidin 二氨基联苯氨
MTT 3’-(4,5-dimethyl-2-thiazoly)2,5-diphenyl- 四甲基偶氮唑蓝
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第三军医大学硕士学位论文
2H-tetrazolium bromide
Cx 26 Connexin 26 缝隙连接蛋白 26
GJIC Gap junction munication 缝隙连接细胞间通讯
HE Hematoxylin and eosin staining 苏木素依红染色
SABC Streptavidin-biotin-plex 链霉素亲合素-生物素复
合物
TUNEL(TDT) Terminal deoxynucleotidyl transferase 末端脱氧核苷酸转化酶
mediated X d UPT nick end labeling
SLDY Scrap loading and dye transfer 划痕标记示踪技术
LY Lucifer yellow CH 罗氏黄荧光染料
HSV-TK Herpes simplex virus thymidine kinase 单纯疱疹病毒胸苷激酶
GCV ganciclovir 丙氧鸟苷
RNAase ribonuclease 核酸酶
L P Log phase 对数期
Triton-100 Poctyl polyethylene glycol phenylether 聚乙二醇辛基苯基醚

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第三军医大学硕士学位论文
Study of ATRA Enhancing the Bystander Effect of
HSV-TK/GCV Gene System on Bladder Cancer

Abstract

Background: Bladder cancer is one of the mon malignant in urinal system.
The curative effect is reduced for the high recur rate after surgery and unsensitivity to the
chemical therapy. It is one of the most important strategies of gene therapies to transfect
HSV-TK into urinal bladder cancers at present, which shows preferable research value and
clinical applying foreground. Many studies have confirmed that the “bystander effect”, that