文档介绍：Rabbit Monoclonal Antibodies
--Better Tools for Better Science
2018/6/4
1
Advantages of RabMAb Technology Over Mouse MAb Technology
Higher quality
High affinity and specificity
Broad applications
High ess rate in meeting customers’ criteria
Novel Epitopes
Recognition of mouse orthologs with high homology to
human antigens
Higher throughput
Stable hybridomas
Multiplex
High humanization probability
Higher homology to human germline sequence than mouse
A in vitro positive proof-of-concept study
2018/6/4
Rabbits Recognize More Epitopes
Mouse
Rabbit
Molecular-weight marker
Fibronectin
Protease digested fibronectin
1 2 3 1 2 3
5 mice and 3 rabbits
Western Analysis
Polysera
Immunized with human fibronectin
2018/6/4
Supernatant From Rabbit Hybridoma Is Highly Active In Western blot
A
B
C
D
(A= 1:5; B= 1:50; C= 1:500; D= 1:1000)
K8
Anti-Human Keratin 8 MAb
2018/6/4
A Random Collection Of RabMAbs Have Extremely High Affinities
Antigen MAb Kon (1/) Koff (1/sec) Kd (M)
ER EPIer39 -3 -9
ER EPIer14 -5 -10
ER EPIer26 -5 -10
ER EPIer38 -4 -9
PR EPIpr42 -2 -8
PR EPIpr33 -6 -12
PR EPIpr88 -4 -10
C29 EPIc2902 -3 -8
C29 EPIc2903 -6 -11
C29 EPIc2905 -4 -9
C29 EPIc2906 -5 -9
Herceptin Kd (M)= x 10-9 $300M
Rituxan x 10-9 $
Synagis x 10-9 $600M
Remicade x 10-10 $270M
2018/6/4
RabMAb For Better Diagnostics
Mouse 1A6
RabMAb
Heat
treated
untreated
2018/6/4
Rabbit MAb
Mouse MAb
Phage MAb
Non Primary Ab
RabMAb: More Accurate Results
--- Comparison of rabbit MAb, mouse MAb and phage MAb
50% false positive
15% false positive
5% false positive
2018/6/4
Fibrin
Degradation
Products
Fibrin (Blood clot)
Plasmin
Plasminogen
Endothelia Cell
PAI-1
tPA
Property
Applicati