文档介绍：抚顺师范高等专科学校
 
 
毕业论文(设计)
 
 
 
 
 
 
论文(设计)题目:羊肚菌培养基及培养条件的研究
学生姓名:陈燕
指导教师:李峰
专业:园艺技术
年级班级:06级生物班
 
2008年12月
摘要
本论文是以名贵菌种羊肚菌为研究对象,通过深层发酵培养对不同培养条件下羊肚菌菌丝生长和胞外多糖生成量进行探索,主要目的就是确定羊肚菌深层培养的培养基配方和最适培养条件,主要方法是通过测定羊肚菌深层培养的菌丝干重和胞外多糖浓度来确定羊肚菌深层培养的最优条件;经过一系列对比研究和正交试验,得到种子培养基配比为:蔗糖3%,%,土豆10%,%,MgSO4 %,%;发酵培养基最适宜的配比是:蔗糖4%,%,麸皮2%,%,%,MgSO4 %。
另外,pH值、摇床转速、接种量、温度也是影响羊肚菌深层培养的重要因素,通过对比实验得出:pH5~6为羊肚菌深层培养最适pH范围;转速在180rpm时生长最好,接种量的影响相对比较小,选定10%接种量;利用固体平皿培养确定最适培养温度为23℃。
关键词:羊肚菌深层培养菌丝多糖
Abstract
In this paper , the noble spawn Morchella esculenta was discussed . The fermented conditions and optimum carbon sources , nitrogen sources and anic salt were studied by studying the submerged fermentation of Morchella esculenta . The two indexes in culturing the dry mycelia and haride yield rate of Morchella esculenta submerged fermentation . The optimum medium for growing seeds were sucrose 3%, peptone %, potato juice10% , potassium dihydrogen phosphate % , magnesium sulfate % , yeast-extract % , and 150ml liquid medium put in 500ml flask .The optimum liquid fermentation medium was listed as follows : sucrose 4% , ammonium nitrate % , wheat bran 2% , yeast-extract % , potassium dihydrogen phosphate % , magnesium sulfate % .
In addition , pH vale、the shaker rotation speed、the amount of inoculumand temperature are all the important elements of influenced Morchella esculenta Submerged fermentation . The results showed that the optimum fermented conditions of Morchella esculenta were ~ , 180r/min , 23℃, inoculum quantity 10%.
Keywords: Morchella esculenta , submerged fermentation, mycela, haride
第一章前言
羊肚菌(Morchella esculenta)又叫美味羊肚菌,是世界公认的一种珍贵的食、药用高等真菌。该菌体味鲜,营养丰富,含有丰富的氨基酸,烟酸、泛酸、生物素等多种维生素及多种微量元素,同时,由于羊肚菌含有多糖这一药用成分,使得它又有很高的药用价值。对羊肚菌的开发研究一直是真菌学家们努力的课题。野生羊肚菌因受气候、海拔、湿度、温度、土壤等环境因素影响,其产量很少,因而价格十分昂贵。要充分开发这一资源,将这一极富保健价值的菌类资源提供广大消费者。
羊肚菌及羊肚菌的发展史
早在《本草纲目》中就有羊肚菜即羊肚菌的记叙:“甘寒无毒、益肠道、化痰理气”。《广菌谱》