文档介绍：浅析川芎嗪对大鼠移植肝的保护功能
:刘胜雄,欧阳永忠,汤明,吴奔腾
【目的探索川芎嗪(TMP)对大鼠移植肝的保护功能。方法参照Delriviere和Kamada等方法建立大鼠肝移植模型,将大鼠随机分为3组:A组(假手术组)、B组(肝移植对照组)和C组(TMP干预组)。采用全自动生化分析仪检测术后血清肝功能(AST、ALT、LDH)水平;应用免疫组化SP法结合病理图像分析仪测定肝组织中Bcl-2、Bax的表达情况;HE染色观察其病理形态学变化。结果(1)C组AST、ALT、LDH明显低于B组(P<)。(2)C组Bcl-2或Bcl-2/Bax表达明显高于B组(P<),而Bax表达无明显变化(P>)。(3)A组无明显病理形态改变,B组术后肝组织出现明显的病理损害,C组病理变化明显减轻。结论 TMP可能通过上调Bcl-2或Bcl-2/Bax表达而减轻肝移植体的低温保存再灌注损伤,对供肝有保护功能。
【肝移植;保存再灌注损伤;川芎嗪;Bcl-2;Bax
Protective effect of tetramethylpyrazine on liver graft in rat liver transplantation
【Abstract Objective To investigate the protective effect of tetramethylpyrazine (TMP) preconditioning on liver graft in rat liver Performing the operation of liver transplantation in male Sprague-Daada osing methods,the rats ly divided into three groups:A group(n=6,sham operation group),B group (n=30
×2,liver transplantation control group),C group (n=30×2,TMP group).The parameters of liver function (AST,ALT and LDH) ical analyser in group B and C after reperfusion ,3h,6h,24h,48h respectively via the plasma samples;part of the liver tissue pled to determine the expression of Bcl-2 and parameters in group A ed 3h after the expression of Bcl-2 and Bax ined by immunohistochemisty technique aging ,parts of liver tissue samples ately normally morphologic structure e obvious hist