黄伞菌丝深层发酵培养条件的研究1.doc

文档介绍：黄伞菌丝深层发酵培养条件的研究
惠丰立,魏明卉,杜敏华,刘征
(南阳师范学院生物系,河南南阳,473061)
摘要:采用摇瓶培养法对黄伞菌丝深层发酵培养条件进行了研究。通过正交试验初步确定黄伞菌丝深层发酵适宜的培养基组成为:葡萄糖3%,%,%,%。该菌株最适培养条件为:培养温度25℃,,接种量15%,发酵周期10d。在优化的试验条件下,进行摇瓶发酵,。
关键词:黄伞;深层发酵;菌丝
中图分类号: 文献标示码:A
Studies on Submerged Fermentation Conditions of Pholiota adiposa
HUI Feng-li,WEI Ming-hui,DU Min-hua,LIU Zheng
(Departmemt of Biology, Nanyang Teachers,College,Nanyang 473061,China)
Astracts: The submerged fermentation conditions of Pholiota adiposa were studied with shake flask culture optimal culture medium obtained through orthogonal designs was as follows: glucose 3%,beef extract %,K2HPO4 %,MgSO4 %.The best cultivation conditions of Pholiota adiposa were : initial pH ,culture temperature 25℃,fermentation time 10d,inoculating amount 15%.Under the optimal conditions, the dry weight of mycelia reached fermentation liquid.
Key words: Pholiota adiposa;submerged fermentation;hyphae
黄伞[Pholiota adiposa(Fr.)Quel.],又名柳蘑、黄蘑、多脂鳞伞,是担子菌纲,伞菌目,球盖菇科,鳞伞属的一种名贵食药兼用真菌。近年来研究发现,黄伞子实体不仅含有丰富的营养物质,如蛋白质、脂肪、纤维素及多种维生素和无机盐外,而且还含有多糖、麦角固醇和胡萝卜素等多种生物活性物质[1]。黄伞活性多糖抗癌作用明显,对小白鼠肉瘤180和艾氏腹水癌的抑制率达80%~90%,还可预防葡萄球菌、大肠杆菌、肺炎杆菌和结核杆菌感染[1,2]。其所含有的麦角甾醇能抑制肿瘤血管的生成,而肿瘤血管生成抑制药物的开发也是当前肿瘤研究的热点和肿瘤治疗的新方向[3,4]。
目前,我国和日本已对黄伞的人工驯化栽培进行了研究,但由于对黄伞的生物学特性了解不够完善,人工栽培子实体,生产周期长,产量低,因而未得到全面开发[1,5,6]。为此,十分有必要对黄伞的深层发酵工艺进行研究,以便用发酵的菌丝体替代子实体作为食品、保健