文档介绍：The Influences of MSC Infused via Different Ways and Different Time on Anti-tumor Activities of CIK/NK cells from Umbilical Cord Blood in K562 NOD/SCID Mice
黎阳吴燕峰王潇娉黄绍良方建培黄科魏菁
中山大学孙逸仙纪念医院儿科
drliyang@
Background
Bone marrow mesenchymal stem cells (MSC) has a unique immunological characteristics:
Low expression of classⅠMHC molecules
Do not express classⅡMHC molecules
Did not express Fas ligand and co-stimulatory molecules
Therefore, MSC is survival possible long-term in the host body, even can be survival long-term in heterogeneity host.
Background
Cytokine-induced killer(CIK) cells are heterogeneous group of cells that obtained from co-cultured mononuclear cells of the human peripheral blood, cord blood or bone marrow with stimulation of variety cytokines such as IL-2, IFN-γ,Anti-CD3 in vitro after a period of time.
CIK cells expressing CD3 and CD56.
Background
Studies have showed that MSC has a negative regulatory role, whether or not its can effect the anti-tumor activity of subsequent infused killer cells in vivo is worth being further studied.
Background
Because CD3+CD56+ CIK cells derived from CD3+CD56- T cells, MSC may also inhibit anti-tumor activity of CD3+CD56+ CIK cells.
Objectives
To explore the influences of MSC infused via different ways and at different time on anti-tumor activities of CIK/NK cells from umbilical cord blood in K562 NOD/SCID mice.
Experimental priniple:
MSC
CIK/NK cells
Inhibition
infusion
infusion
K562 NOD/SCID mice
Shorten survival
Increase in tumor burden
Experimental groups:
NOD/SCID mice
MSC CIK/NK
Group A
at the same time
(caudal vein) (caudal vein)
Group B
Delayed 48h
MSC CIK/NK
(caudal vein) (caudal vein)
Group D
Group C
MSC CIK/NK
(marrow cavity) (caudal vein)
at the same time
Delayed 48h
MSC CIK/NK
(marrow cavity) (caudal vein)
Group E
CIK/NK
(caudal vein)
Group F
Delayed 48 hours
CIK/NK
(caudal vein)
Group G
K562 cells (inoculated alone)
K562
After 12h
After 12h
Detection