文档介绍：玻璃化冷冻方法在兔胚胎冷冻保存中的应用
摘要
本文通过兔胚胎毒性实验和玻璃化冷冻的研究,确定对胚胎无毒性或毒性小的冷
冻保护剂的种类、最佳浓度、胚胎发育阶段,为玻璃化冷冻方法在兔胚胎冷冻保存中
的应用提供理论基础。
%%。经统计分析,
兔早期囊胚存活率海藻糖组显著高于蔗糖组(P<)。
,分别用OM(对照组)、、、
理兔早期囊胚,%、%、%、%。经统计分析,对照
组与不同浓度甘油组之间兔早期囊胚的存活率均无显著性差异(P>)。
,兔早期囊胚和桑椹
%、%。经统计分析,早期囊胚的存活率极显著高于桑椹
胚(P<)。
(EG)对兔胚胎冷冻效果的影响实验中,解冻回收后正常胚胎
数和囊胚孵化率,对照组与IO%EG、20%E6组之间无显著性差异(P>),但对照组、
IO%EG、20%EG组的解冻回收后庀常胚胎数和囊胚孵化率均极显著高于30%EG组
(P<)。
,体内胚胎和体外胚胎的
%、%;%%。经
统计分析,解冻回收后正常胚胎数体内胚胎组显著高于体外胚胎(P(),囊胚孵
化率两组之间无显著性差异(P>)。
,胚胎解冻回收后『F常胚胎数和
囊胚孵化率,缓慢冷冻法与玻璃化冷冻法之间均无显著性差异(P>)。
关键词:兔胚胎冷冻保护剂玻璃化冷冻
II
Application of Rabbit Embryos Cryopreservation by Vitrification
Tian Jiaming
Directed咖Li Zhongshu

and

Hong

Chunxi
ABSTRACT

These experiments were carried

OUt

tO

clarify the kinds,the best
concentration of the cryoprotectant,which has

no

or

little toxicity

to

embryos,as well
as

the suitable stage of embryo

development by

studying

on

the toxicity and
vitrification of the rabbit,SO that offered theory base for the application of rabbit
embryos cryopreservafion by vitrification.
survival rates of rabbit early blaStocyst treated with trehalose and

sucrose
were

%and %.

groups treated with trehalose were
significantly higher

than

those that treated wi山sucrose

rP<).

survival

rates

of rabbit early blaStocyst,which added trehalose

tO
(contr01),, and

glycerol,were %、
%、%and %. were

not

significantly

different
between controls and others(P>).
3.。The survival rates of rabbit early blastocyst and marula were
%and

%.
respect