文档介绍：当代医学 2012年9月第18卷第25期总第288期 Contemporary Medicine, Sep. 2012, Issue
doi:.1009-
桑叶药材水浸出物和指标成份的含量测定研究
李克雄高崇凯
[摘要] 目的研究桑叶药材的质量,通过药材提取物(流膏、干膏和浸出物)和指标成份含量的测定以及药材的薄层鉴别,从定量角度来研究药
材质量。方法主要采用高效液相色谱法,十八烷基硅烷键合硅胶为填充剂,甲醇-乙腈-醋酸-水(15:10::)为流动相,检测波长为358nm,流速
1mL/min,柱温30℃。结果不同产地5批桑叶原药材、流浸膏和干膏各样品之间的芦丁含量具有明显的差异,即便是同一产地不同批号的样品芦丁含量
也存在很大的差别。其原药材、流浸膏和干膏芦丁含量的大小次序是一致的,%、%%,湖北2最低分别
%、%%。浸出物的含量测定亦有差异,但均符合标准。水分测定几乎无差异符合规定。结论通过对桑叶药材浸出物和指标成份的
含量测定,可从定量的角度更好地对药材质量进行控制。
[关键词] 桑叶;浸出物;高效液相色谱法;含量测定
[Abstract] Objective To study the quality of medicinal herbs of Mulberry Leaf. The quality of medicinal herbs is studied quantitatively by
determining the content of extracts ponent indices of Mulberry Leaf medicinal herbs and TLC of the herbs. Methods The chromatographic
separation was achieved on a Diamonsil ODS-C18 column with amobile phase of methanol-acetonitrile-aceticacid-water solution(15:10::). The
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药物与临床ﬂ ow rate was and the column temperature was 30℃. Results The differences can be seen obviously among 5 batches of sample of rutin
content in origin mulberry leaf raw herbs, ﬂ ow paste and dry extract from different places. Additionally, though the samples of rutin content of different
batches from the same origin, there is also a big difference. Th