文档介绍：毕业设计(论文)
题目基于云南臭蛙抗菌肽序列特性
分析的药物设计策略
学院(部) 生物产业学院
专业药学
学生姓名
学号 200710516105 年级 07级
指导教师职称讲师
2011 年 5 月 27 日
基于云南臭蛙抗菌肽序列特性分析的药物设计策略
摘要:[目的]分析抗菌肽结构与活性之间的关系,提出一种抗菌肽类药物的设计策略,为以后抗菌肽分子的设计和改造提供理论基础。[方法]采用生物信息学的方法,分别从云南臭蛙抗菌肽的一级结构、二级结构、导肽、信号肽、功能域、保守结构域、疏水/亲水性分析和多序列比对等方面对9种云南臭蛙抗菌肽进行序列特性分析,并以这些抗菌肽序列为模板进行设计和改造。[结果]云南臭蛙成熟肽均为带正电荷的疏水性蛋白,α螺旋是其二级结构的主要元件,该类肽的C端均具有保守的Rana-box环状结构。在此基础上,人工合成了一条可能具有抗菌活性的序列:GL(A/V/I)AANFLPK(T)AF(L)C
XXXK(R)KC。[结论]α螺旋和正电荷是保证抗菌肽具备抗菌活性的主要条件,在对成熟肽序列进行氨基酸残基替代或肽链截短后,可化学合成此类抗菌肽,为此类药物的获得提供一条捷径。
关键词:云南臭蛙;抗菌肽;生物信息学;药物设计策略
Drug design strategy based on sequence characteristic analysis of Rana andersonii antimicrobial peptides
Abstract:[Objective] To analyze the relationship between the structure and activity of antibacterial peptides, put forward a design strategy of antibacterial peptide drugs, providing a theoretical basis for the future design and modification of the peptide molecules. [Method] Nine kinds of Rana andersonii antibacterial peptides were analyzed by using the bioinformatics tools, including the structure of primary and secondary, target and signal peptide, functional domain, conserved domain, hydrophobic/hydrophilic and multiple sequence alignment. [Results]Mature peptides from Rana andersonii are positively charged hydrophobic protein and α-helix is the ponent of their secondary structure. Such peptides all have conservative Rana-box ring structure on their C-terminal. Based on the results, a peptide: GL (A/V/I) AANFLPK (T)AF(L)CXXXK(R)KC may have strong activity was synthesized artificially. [Conlusion]α-helix structure and positive charge of the peptide are the main factors to the peptides’ activity. It is a shortcut to obtain the peptide like drugs by amino acid residue substitution or peptide chain truncation.
Key words: Rana andersonii; Antibacterial peptide; Bioinformatics; Drug design strategy
目录
绪论…………………………………………………………………………………………………………1
第1章材料与方法…………………………………………………………………………………………3
材料………………………………………………………………………………