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5. Protein Assays and Error Analysis
. Competent Chemist Rating: “What's in a Cow's Heart Anyway?”
Techniques Checklist:
• Pipetting with pipetman ❑
• Calibrating pipetman ❑
• Preparation of a standard curve ❑
• Serial dilution ❑
• UV-Vis Spectroscopy ❑
Pre-Lab:
• Discussion of Protein Assays
Equipment:
• Pipetman: 100 P, 1000 P
• Pipet tips - large and small
• 8 Test tubes
• Eppendorf tubes and holder
• Disposable UV-Vis cuvettes - 5 mL
Goal:
• You will be given a sample of a solution of bovine heart cytochrome c. You will
use the Coomassie® Plus Protein Assay from Pierce to determine the concentration of
protein in the sample.
Note:
• You will receive a tray of Eppendorf tubes: one containing stock solution, three
containing 50 µL each of bovine heart cytochrome c, and several empty tubes for mixing
solutions. You will also be provided with a bottle of 2