文档介绍：Gene Manipulation Medical ics bination DNA Technique Techniques for joining DNA molecules in vitro and introducing them into living cells where they replicate. Insert specific DNA into vector Clone in host cells Cloning Isolate cloned DNA foreign DNA bacterial cell Schematic of bination DNA technique vector restriction endonuclease foreign DNA bacterial cell Schematic of bination DNA technique ligase vector restriction endonuclease foreign DNA bacterial cell host Schematic of bination DNA technique Schematic of bination DNA technique Cloning Amp Amp+X-gal Schematic of bination DNA technique “Blue-white” screening bination DNA DNA clone cDNA clone Artificial gene clone PCR product clone Gene Transfer Transgenic animal Transgenic mouse Embryonic stem cell,ES cell