脑和脊髓保护研究课件.ppt

文档介绍：脑和脊髓保护研究
第四军医大学西京医院
熊利泽
研究?
研究方向
动物模型
切入点?
国际公认的动物模型:
局灶脑缺血大鼠模型
脊髓缺血大白兔模型
评估方法或指标简便、实用
神经行为学评估(计分法)
脑梗死容积大小--TTC染色
正常神经元计数--HE染色
Parameters
MCAO Rat
Neurologic Deficit Scores
偏瘫的程度
Infarct Volume
梗死灶的大小
Neurologic Deficit Scores
0: no deficit
1: failure to extend left forepaw fully
2: circling to the left
3: falling to the left
4: no spontaneous walking, with a
depressed level of consciousness
5. stroke-related dead
Infarct Volume
Rat Brain
Iced saline
10min
Coronal sections
(2mm)
2%TTC
10%Formalin
37℃ 30min
Photograph,analysis
24h
1
2
3
4
Parameters
Spinal Cord Ischemia Rabbit
Hind-limb Motor Function Scoring

Number of Normal Motor Neurons
Hind-limb motor function Score (Tarlov Criteria)
0: no voluntary hind-limb movement
1: movement of joints perceptible
2: active movement but unable to stand
3: able to stand but unable to walk
4: complete normal hind-limb
motor function