文档介绍：Molecular Biology of the Gene, 5/E --- Watson et al. (2004)
Part I: Chemistry and ics
Part II: Maintenance of the Genome
Part III: Expression of the Genome
Part IV: Regulation
Part V: Methods
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Part II: Maintenance of the Genome
Dedicated to the structure of DNA and the processes that propagate, maintain and alter it from one cell generation to the next
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CHAPTER 3: The replication of DNA (DNA的复制)
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The revised central dogma
Translation
RNA processing
DNA repair
基因组的保持
基因组的表达
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1. The Chemistry of DNA Synthesis(DNA合成的化学基础)
2. The Mechanism of DNA Polymerase(DNA聚合酶的作用机制)
3. The Specialization of DNA Polymerases(DNA聚合酶的特化)
4. The Replication Fork(复制叉)
5. DNA Synthesis at the Replication Fork(复制叉上的DNA合成)
6. Initiation of DNA Replication(复制的起始)
7. Binding and Unwinding(结合和解旋)
8. Finishing Replication(复制的终止)
Outline
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The first part describes the basic chemistry of DNA synthesis and the function of the DNA polymerase
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1. The Chemistry of DNA Synthesis
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1 DNA synthesis requires deoxynucleoside triphosphates and a primer:template junction
(DNA合成需要脱氧核苷三磷酸和引物-模板接头)
DNA合成必须具备的两个关键底物:
四种脱氧核苷三磷酸-dATP, dGTP, dCTP, dTTP
2. 引物-模板接头( primer:template junction)
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Substrates required for DNA synthesis
2‘-脱氧核糖核苷三磷酸
退火引物
DNA的生长末端
模板链
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DNA is synthesized by extending the 3’ end of the primer
(DNA通过引物3’端的延伸进行合成)
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