文档介绍：大孔吸附树脂分离纯化迷迭香中迷迭香酸
的研究
【摘要】目的考察大孔吸附树脂对迷迭香酸的吸附性能和纯化效果,寻找从迷迭香中分离纯化迷迭香酸的较优工艺。方法以迷迭香酸的吸附量、解吸率和所得粉末中迷迭香酸的含量为指标,从4种大孔吸附树脂中筛选出较好的D101 树脂。通过静态和动态实验,对迷迭香酸在D101树脂上吸附和解吸的条件进行考察和优化。结果采用D 101树脂从迷迭香中分离纯化迷迭香酸的较优工艺参数为:上柱液PH3〜 4,速度2BV/h,溶液处理量9BV,洗脱剂为40%乙醇,洗脱速度lBV/h,收集洗脱液3BV。按此工艺条件,迷迭香酸的解吸率为%,3BV洗脱液浓缩干燥后,所得粉末中迷迭香酸含量为%,树脂经8次重复使用,吸附量和分离效果无明显下降,可以重复使用。结论D10 1树脂可从迷迭香中较好的分离纯化迷迭香酸,具有潜在的工业应用价值。
【关键词】迷迭香酸;大孔吸附树脂;迷迭香;分离;
纯化
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迷迭香Rosmarinusofncina
物,为多年生常绿小灌木,原产于地中海地区,国内近年来大面积的引种栽培。该植物具有抗氧化、抗病毒和抗肿瘤等活性,广泛应用于医药、食品和饮料等行业。研究发现[1〜 4]:该植物所含的水溶性酚酸成分一迷迭香酸具有明显的抗氧化、抗炎、免疫抑制、抗血栓、抗菌、抗病毒等生物活