文档介绍：足叶乙苷脂质体包封率测定方法研究
时军1,陈伟鸿2,程怡2,林德晖2
(,广东药学院中药学院,510006;,广州中医药大学中药学院,510006)
摘要目的建立足叶乙苷脂质体的包封率测定方法。方法采用乙醇注入法制备足叶乙苷脂质体,以葡聚糖凝胶分离-高效液相色谱法测定含量和包封率。结果洗脱曲线表明采用蒸馏水()作为洗脱溶剂,%,同一批样品包封率测定结果RSD在2%以内。结论葡聚糖凝胶分离-高效液相色谱法操作简单、准确、重复性好,可用于足叶乙苷脂质体的包封率的测定。
关键词足叶乙苷脂质体包封率
Study on Entrapment Efficiency Determination of Etoposide Liposome
Shi Jun1 Chen Weihong2 Cheng Yi2 Lin Dehui2
( Pharmaceutical University , University of Chinese Medicine)
Abstract Objective To establish encapsulation efficiency determination method of etoposide liposome. Methods To prepare etoposide liposome by using ethanol injection method, use gel filtration to determine the content and entrapment efficiency of etoposide bining high-performance liquid chromatography. Results The elution curve shows that the use of distilled water (flow rate ) as the eluting solvent, column recovery ratio is %, encapsulation efficiency RSD of the same sample is within 2%. Conclusion Gel bining high-performance liquid chromatography method is simple, accurate, reproducible, and it can be used to determine entrapment efficiency of etoposide liposomes .
Key Words Etoposide Liposome Entrapment efficiency
足叶乙苷是临床治疗小细胞肺癌、宫颈癌、白血病的一线药物[1],但存在水溶性差等问题,其注射剂毒副作用大、药物分布广泛。为提高足叶乙苷抗癌效果,将其设计制成脂质体制剂,以期增加治疗指数,延长体内的滞留时间。为有效控制足叶乙苷脂质体的质量,采用凝胶柱色谱分离结合HPLC法测定包封率,结果表明