文档介绍：丹参酮ⅡA对阿霉素所致大鼠心肌损伤的保护作用
[摘要] 目的观察丹参酮ⅡA对阿霉素所致大鼠心肌损伤的保护作用及可能机制。方法将SD大鼠随机分为对照组、模型组(阿霉素心脏损伤)、丹参酮ⅡA组(×10-5、×10-5、×10-5 mol/L)。丹参酮ⅡA组大鼠造模前给予20 mL/kg体积丹参酮ⅡA混悬液灌胃,连续5 d,第5天给药后1 h腹腔注射18 mg/kg阿霉素溶液,此后继续灌胃2 d。检查大鼠的心脏功能,检测大鼠血浆乳酸脱氢酶(LDH)、肌酸激酶(CK)、丙二醛(MDA)、超氧化物歧化酶(SOD)水平和心肌组织中肌钙蛋白Ⅰ(cTnⅠ)水平。结果与模型组相比,丹参酮ⅡA组(×10-5、×10-5 mol/L)明显改善心脏功能,表现为左室收缩压[(±)mm Hg比(±)、(±)mm Hg,F=]、左室压力差[(±)mm Hg比(±)、(±)mm Hg,F=]、左室内压最大上升速率[(±)mm Hg/s比(±)、(±)mm Hg/s,F=]和左室内压最大下降速率[(±)mm Hg/s比(±)、(±)mm Hg/s,F=]均显著性升高(均P < )。与模型组比较,丹参酮ⅡA(×10-5、×10-5 mol/L)显著降低了心肌组织匀浆中cTnⅠ[(±)ng/mgpr比(±)、(±)ng/mgpr,F=]、LDH[(±)U/L比(±)、(±)U/L,F=]、CK[(±)U/mL比(±)、(±)U/mL,F=]和MDA[(
±)μmol/mL比(±)、(±)μmol/mL,F=]的水平,增加了SOD活性[(±)kU/L比(±)、(±)kU/L,F=](均P < )。结论丹参酮ⅡA对阿霉素诱导的大鼠心肌损伤具有保护作用,其机制可能是丹参酮ⅡA抑制氧化应激有关。
[关键词] 丹参酮ⅡA;阿霉素;心肌损伤;氧化应激
[中图分类号] [文献标识码] A [文章编号] 1673-7210(2014)08(c)-0025-04
Protective effect of Tanshinone ⅡA on the cardiac injury induced by Adriamycin in rats
CAO Jiehong1 XU Ninghong1 TAN Huaqing2
of Oncology, the Central Hospital of Loudi City, Hu'nan Province, Loudi 417000, China; of Cardiology, the Central Hospital of Loudi City, Hu'nan Province, Loudi 417000, China
[Abstract] Objective To investigate the protective effect of Tanshinone ⅡA on the cardiac injury induced by Adriamycin and explore the probalble mechanism in rats. Methods The SD rats were randomly divided into control group, model group (Adriamycin-induced cardiac injury) and Tanshinone ⅡA group (×10-5, ×10-5, ×10-5 mol/L). Tanshinone ⅡA (20 mL/kg) was administered by lavage every day, in the fifth days, Adriamycin (18 mg/kg) was administered by intraperitoneal in