文档介绍：第 33 卷第 1 期浙江师范大学学报(自然科学版) .33, .1
2010 年 3 月( . .) Vol .20N1o0
Journal of Zhejiang Normal University Nat Sci Mar
文章编号:1001-5051(2010)01-0089-06
磁
应用分子标记探讨酵母菌种间亲缘关系
ISSR
徐晓波, 蒋冬花, 李杰, 后家衡
(浙江师范大学化学与生命科学学院,浙江金华 321004)
摘要:从 27 条简单重复序列区间( )引物中筛选出 10 条引物对 19 株酵母菌进行扩增;采用正交
ISSR 2 + PCR
试验设计方法,对的影响因素进行了研究,对酶, , 和引物进行了 4 因素 3 水平优化试验,
ISSR Taq Mg dNTP
再通过对退火温度和模板浓度的筛选,得出- 条引物共扩增出108条
DNA ISSR PCR
带,其中 %呈多态性,相似系数为 ~.
关键词:简单重复序列区间;酵母菌;正交设计;聚类分析
中图分类号: 文献标识码:
Q A
ic diversity analysis with ISSR PCR on different yeasts
, , ,
XU Xiaobo JIANG Donghua LI Jie HOU Jiaheng
(College of Chemistry and Life Science, Zhejiang Normal University, Jinhua Zhejiang 321004, China)
Abstract: 27 19
Ten primers selected from ISSR primers were used for ISSR amplification of tested yeast
. ( -
strains The orthogonal design was used to optimize ISSR amplification system in four factors Taq poly
, 2 +, , ) , -
merase Mg dNTP primer at three levels respectively ISSR amplification system was optimized in an
. 108 10 , -
neal temperature and template DNA concentration A total of bands were produced with primers a
% , .
mong which were polymorphic similarity coefficient ranged from to
Key words: - ( ); ; ;
inter simple sequence repeat ISSR yeast orthogonal design cluster analysis
酵母菌是一类十分重要的微生物,对其分类与鉴定是人类开发利