文档介绍：294乙型肝炎病毒感染者外周血T淋巴细胞亚群表达与其血清HBV-DNA水平的相关性分析范公忍1,赵满仓2,魏文清2,李娟‘,贾志凌2,王北宁2(北京军区总医院,,,北京100700)摘要:观察乙型肝炎病毒(HBV)感染者外周血T细胞亚群功能变化与其血清HBV-DNA水平,探讨病毒复制程度与细胞免疫功能的关系。应用流式细胞术直接荧光法检测142例HBV感染者和35名健康对照者外周血中T淋巴细胞亚群百分率,用荧光定量PCR法检测HBV感染者血清HBV-DNA。结果显示:与健康对照组比较,慢性乙型肝炎组CD4+细胞数量降低,CD8+细胞数量明显升高(P均<),肝硬化组CD4+/CD8+细胞比值与健康对照组比较明显降低,CD4+CD25+调节性T细胞数量明显增加(P<)。结论:慢性乙型肝炎患者可导致体内细胞免疫功能紊乱,HBV-DNA高复制状态可进一步促进细胞免疫功能紊乱,CD4+/CD8比值的动态检测可为指导临床免疫治疗提供依据。关键词:慢性乙型肝炎;T细胞亚群;流式细胞术;血清HBV-DNA中图分类号:R521文献标识码:A文章编号:1006—1703(2011)05-0294·04RelationshipBetweenSerumHBV-DNAReplicationandTLymphocyteSubsetsofPeripheralBloodinPatients、).rithHepatitisBFANGong-ren,ZHAOMan-cang,WEIWen-qing,LIJuan,JIAZhi-ling,WANGBei-ninghematologyandCentrallaboratory,mandBeijingl007∞,China)Abstract:ToobserveTlymphocytesubsetsinperipheralbloodinpatientswithhepatitisBandthereplicationofser-ulnhepatitisBvirus(HBV-DNA),toexploretherelationshipbetweentheviralreplicationdegreeandcellularim--DNAwasmeasuredbyfluorescencequantitativepolymeraaechainreaction(PCR).Comparedwithhealthycontrols,thepercentageofCD4+Tcellsinpatients稍t}lchronichepatitisBweresignificantlydecreasedandthepercentageofCD8+Tcellswereincreased(P<).Comparedwithhealthycontrols,theCD4+/CD6+ratioinpatientswithcirrho