文档介绍：134例自身免疫性肝病患者自身抗体的分析
天津市第三中心医院        周淑芬 刘树业   丁贤
     摘要目的:探讨自身免疫性肝脏疾病的自身抗体特征,以提高对该病的认识。方法:在134例患者中,原发性胆汁性肝硬化(PBC)73例,自身免疫性肝炎(AIH)43例,原发性硬化性胆管炎(PSC)4例,乙型病毒肝炎(HBV)3例,丙型病毒肝炎(HCV)6例,非甲~戊型病毒肝炎(Non A-E)5例。采用间接免疫荧光法、免疫印记法等分别检测抗核抗体(ANA)、抗线粒体抗体(AMA)、抗肝肾微粒体抗体Ⅰ型(LKM-1)、抗可溶性肝抗原/肝胰抗原(SLA/LP)和AMA-M2亚型等。结果:6例LKM-1阳性,6例SLA/LP阳性,分别见于AIH、AIH/PBC重叠综合征和HCV患者。%,%,以胞浆型为主(32/66)。AIH患者,%,以核膜型为主(19/39)。非甲~戊组,2例AMA和AMA-M2阳性,1例SMA阳性。结论:检测自身抗体有助于该病的诊断,临床不能忽视肝功能异常的患者,诊断时应考虑可能患有自身免疫性肝病。
    关键词肝炎,自身免疫性自身抗体免疫印迹法荧光抗体技术,间接
   Detection of Autoantibodies in patients with Autoimmune Liver Disease
     ZHOU Shufen ,LIU Shuye  DING Xian(TheThird Central Hospital of Tianjin 300170)
    Abstract  Objective: To study the characteristics of autoantibodies in autoimmune liver diseases and to improve its :One hundred and thirty four patients were included in this study,  Among them 73 patients with primary biliary cirrhosis (PBC) , 43 patients with autoimmune hepatitis(AIH) , 4 patients with primary sclerosing cholangitis (PSC) , 3 patients with hepatitis B (HBV), 6 patients with hepatitis C(HCV) and 5 patients with hepatitis non AE(Non A-E) . Anti-nuclear antibodies(ANA),anti-mitochondral antibodies(AMA),anti-smooth muscle antibodies(SMA)and anti-liver kidney microsomal antibody(LKM-1)were tested by indirect immunofluorescence .LKM-1,soluble liver anti