中国47 个小麦新品种(系) 苗期抗叶锈基因推导.doc

文档介绍：中国47个小麦新品种(系)苗期抗叶锈基因推导
袁军海1,2,3,刘太国1,陈万权1
(1中国农业科学院植物保护研究所植物病虫害生物学国家重点实验室,北京 100094;2中国农业大学农学与生物技术学院,北京 100094; 3河北北方学院农学系,宣化 075131)
摘要:【目的】探明中国47个小麦新品种(系)携带的苗期抗叶锈基因状况,改进和完善基因推导方法。【方法】选用17个具有较高鉴别能力的致病类型,在不同温度和(或)光照强度下测定,结合系谱分析进行基因推导。【结果】在供试的47个小麦品种(系)中,推导出Lr1(存在于11个品种或品系中)、Lr3(7)、Lr3bg(3)、Lr9(3)、Lr10(3)、Lr13(10)、Lr16(6)、Lr23(2)、Lr26(14)和Lr34(1)共10个已知抗病基因,另有42个品种(系)含有未知基因。【结论】在苗期进行基因推导时,尽量多地选择鉴别能力强的致病类型,在相对稳定均一的环境条件下重复测定,并结合系谱分析,才能获得可靠的结果。结合温度与光照强度梯度,具有持久抗病性潜质的抗叶锈基因Lr13和Lr34可在苗期进行推导。
关键词:小麦;叶锈;抗病基因;基因推导;成株期抗病性
Postulation of Leaf Rust Resistance Genes in 47 New Wheat Cultivars (Lines) at Seedling Stage
YUAN Jun-hai1,2,3, LIU Tai-guo1, CHEN Wan-quan1
(1State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100094; 2College of Agriculture and Biotechnology, China Agricultural University, Beijing 100094; 3Department of Agronomy, Hebei North University, Xuanhua, Hebei, 075131)
Abstract: 【Objective】 The objectives of this study were to ascertain leaf rust resistance genes in 47 new wheat cultivars (lines) at seedling stage, and to consummate the methods of gene postulation. 【Method】Seventeen pathotypes of inia triticina under the different temperatures and (or) light intensity conditions and pedigree analysis were used for the postulation of leaf rust resistance genes in the tested cultivars (or lines).【Result】Ten named genes, Lr1 (in 11 cultivars or lines), Lr3 (7), Lr3bg (3), Lr9 (3), Lr10 (3), Lr13 (10), Lr16 (6), Lr23 (2), Lr26 (14), and Lr34 (1), were identified. Unknown genes were found in forty-two cultivars (lines). 【Conclusion】The study suggested that Lr13 and Lr34, two potentially durable resistance genes, could be postulated at the seedling stage with different temperature and light intensity, and reliable results could only be obtained from more differential pathotypes, repeated tests under relatively steady and uniform environment, combined with pedigree an