文档介绍：NF-κB、S100B在新生儿脑损伤中的早期诊断价值
赵建美
(南通大学附属医院儿科,江苏南通 226001)
摘要目的检测母妊娠期高血压疾病儿脐带组织核转录因子kB(NF-kB)、S100B的表达及脐血S100B水平,探讨NF-kB、S100B在母妊娠期高血压疾病儿脑损伤中的早期诊断价值。方法将97例母妊娠期高血压疾病所生新生儿纳入实验组,并据其生后第3~7天或纠正胎龄40周后3~7天的20项新生儿神经行为测定(NBNA)评分分为两组:≤35分为脑损伤组,>35分为非脑损伤组;同时选取20例健康母亲所生正常新生儿作为对照组。回顾性分析其脐带组织NF-kB、S100B的表达及脐血S100B水平。免疫组化法检测脐带组织NF-kB、S100B表达;酶联免疫吸附法( ELISA)检测脐血S100B蛋白。对照组、非脑损伤组及脑损伤组间脐带组织NF-kB、S100B和脐血S100B数值比较采用单因素方差分析;NF-kB、S100B与NBNA间相关性分析用Pearson相关系数表达。绘制ROC曲线并计算相应的曲线下面积,以评估NF-kB、S100B对母妊娠高血压疾病儿脑损伤的早期诊断价值。结果 1. NF-κB在脐带组织存在表达,脑损伤组与非脑损伤组脐带组织NF-κB表达无显著性差异(p>),但两组脐带组织NF-κB表达与正常对照组间均有显著性差异(p<)。2. S100B在脐带组织存在表达,脑损伤组、非脑损伤组及正常对照组间脐带组织S100B表达无显著性差异(p>);脑损伤组脐血S100B蛋白水平较非脑损伤组显著增高,非脑损伤组脐血S100B蛋白水平也较正常对照组间显著增高(p<)。3. 母妊娠高血压疾病儿脐血S100B蛋白水平与NBNA评分呈负相关,相关系数分别为- (p<)。 4. ,,诊断脑损伤的特异度94%、%。结论 1. NF-κB作为转录因子,参与了母妊娠高血压疾病儿缺氧缺血性脑损伤的发生、发展。2. 脐血S100B蛋白水平基本不受脐带组织S100B蛋白表达的影响,脐血S100B蛋白对早期诊断母妊娠高血压疾病儿缺氧缺血性脑损伤具有特异性价值。
关键词: 妊娠高血压疾病; S100B;核转录因子kB;脑损伤
Significance of Nuclear Factor kappa B ,S100B protein for early diagnosis of brain damage in neonates ZHAO Jianmei Department of Pediatrics, Affiliated
Hospital of Nantong University, Jiangsu Nantong 226001
Abstract Objective To observe the significance of nuclear factor kappa B
基金项目:南通大学附属医院院级课题
作者简介:赵建美(1971-),女,副教授,副主任医师,硕士生导师。E-mail:zjmheart@
(NF-kB),S100B protein in umbilical cord and S100B protein in cord blood for early diagnosis of brain damage in neonates whose mothers had hypertensive plicating pregnancy. Methods 97 neonates whose mothers had hypertensive plicating pregnancy were selected as case group and they were performed Neonatal Behavioral Neurological Assessment (NBNA) at the age of 3~7 days of full-term newborns or the corrected age of preterm infants. According to NBNA scores, they were divided into brain damage group (NBNA scores≤35) and non-brain damage group (NBNA scores>35). 20 healthy neonates were selected into healthy control group at the same time.