SELDI-TOF-MS 联合LCM 技术筛选大肠癌肝转移早期诊断标志蛋白.pdf

文档介绍：® 世界华人消化杂志 2012年2月8日; 20(4): 296-303
wcjd@ ISSN 1009-3079 (print) ISSN 2219-2859 (online)
临床研究 CLINICAL RESEARCH
SELDI-TOF-MS联合LCM技术筛选大肠癌肝转移早期诊
断标志蛋白
赵渊博, 王云海, 阿不都外力·吾守尔
■背景资料赵渊博, 王云海, 阿不都外力·吾守尔, 新疆医科大学第一 ware and identified by querying the database.
SELDI-TOF-MS 附属医院胃肠外科新疆维吾尔自治区乌鲁木齐市 830054
是集蛋白分离、赵渊博, 硕士, 主要从事大肠癌蛋白质组学的研究.
纯化、数据处理作者贡献分布: 本研究的主要实验、数据分析和文章起草由赵 RESULTS: Fifteen differentially expressed pro-
为一体的蛋白组渊博完成; 病例选择、样本取材及临床资料整理由王云海完成; teins were found between primary tumor cells

学技术, 其敏感课题设计、文章修改和审阅由阿不都外力·吾守尔完成. and normal colorectal cells, of which 12 were
性、特异性高. 通讯作者: 阿不都外力·吾守尔, 副主任医师, 830054, 新疆维
吾尔自治区乌鲁木齐市鲤鱼山南路137号, 新疆医科大学第一附 up-regulated and 3 down-regulated. Nine dif-
LCM是从组织中
属医院胃肠外科. 93978258@
纯化细胞最好的 ferentially expressed proteins were found be-
电话: [phone] 传真: [phone]
方法之一, 可为蛋 tween liver metastatic cells and primary tumor
收稿日期: 2011-11-21 修回日期: 2012-01-04
白组学研究提供
接受日期: 2012-02-06 在线出版日期: 2012-02-08 cells, of which 5 were up-regulated and 4 down-
最佳的生物样本. regulated. Twenty differentially expressed
proteins were identified by querying ExPasy
Application of SELDI-TOF-MS protein database, including integral membrane
and LCM to screen protein protein 2C, DNA repair protein RAD51 homolog
4, cell cycle checkpoint protein RAD1, human
markers for early diagnosis of epididymis protein 4, centromere protein R