文档介绍：临床肝胆病杂志 2011年第27卷第4期 391
磷脂酰肌醇蛋白聚糖-3和甲胎蛋白联合检测
对原发性肝癌的诊断价值
宋孟锜,杨永飞,王冬冬,由法平,陈立波
(华中科技大学同济医学院附属协和医院肝胆外科,武汉 430022)
摘要: 目的通过联合检测磷脂酰肌醇蛋白聚糖-3(GPC3)、甲胎蛋白(AFP) 在原发性肝癌患者血清及组织中的表达情况,
探讨对原发性肝癌的诊断价值。方法分别采用ELISA和免疫组织化学法检测57例肝癌、74例肝炎后肝硬化患者和47例正常血
清和肝组织GPC3、AFP表达水平,根据不同临床病理指标进行分组比较。结果(1)肝癌患者、肝炎后肝硬化及正常对照者血清
中GPC3水平分别为(±)、(±)、(±)ng/ml;肝癌患者血清GPC3浓度显著高于正常及肝炎后肝硬化患
者(t=,P<;t=,P<);血清GPC3、%%,均显著
高于任一单项检测(t=,P<;t=,P<);(2)GPC3在肝癌组织表达高于癌旁和正常肝组织(t=,P<;
t=,P<);GPC3与肿瘤大小、肿瘤数目、HBsAg及AFP水平无明显相关,而与病理分级和临床分期有关;(3)AFP阳
%,%。结论 GPC3联合AFP检测有助于提高原发性肝癌
的确诊率,检测GPC3有助于提高AFP阴性患者肝癌的确诊率。
关键词: 肝肿瘤;磷脂酰肌醇蛋白聚糖类;甲胎蛋白类
中图分类号: 文献标识码: A 文章编号: 1001-5256(2011)04-0391-04
The diagnostic role bination of glypican-3 and AFP in primary hepatic carcinoma
SONG Meng-qi, YANG Yong-fei, WANG Dong-dong, et al. (Department of Hepatobiliary Surgery, Union Hospital, Tongji Medical
College, Huazhong University of Science and Technology, Wuhan 430022, China)
Abstract: Objective To detect the expression of Glypican-3(GPC3) and α-fetoprotein (AFP) in serum and tissues of hepatic carcinoma
patients. Methods ELISA and immunohistochemical staining were applied to detect GPC3 and AFP expressing level in the serum and
tissues in 57 cases with primary hepatic carcinoma