文档介绍：中国农业科学
2012,45(19):3909-3920
Scientia Agricultura Sinica
doi: .0578-
多环境条件下大豆异黄酮主要组分的 QTL 定位
张晶莹,葛一楠,孙君明,韩粉霞,于福宽,闫淑荣,杨华
(中国农业科学院作物科学研究所/农业部北京大豆生物学重点实验室北京 100081)
摘要:【目的】利用不同定位方法,对不同环境条件下大豆异黄酮主要组分进行 QTL 定位研究,为大豆异黄
酮分子标记辅助育种提供理论依据。【方法】以异黄酮含量有显著差异的鲁黑豆 2 号(3 μg·g-1)和南汇早
黑豆(1 μg·g-1)为亲本构建的 F5∶7-8 重组自交系为材料,分析 RIL 群体的 SSR 标记多态性,结合 HPLC 法
鉴定异黄酮主要组分含量。【结果】绘制了一张包含 161 个多态性 SSR 标记,全长 3 cM 的大豆遗传连锁图
谱。利用 ICIMapping 软件的 ICIM、IM 和 SMA 3 种定位方法,共定位到 4 种环境下与异黄酮主要组分相关的
14 个 QTL。【结论】3 个标记区间在多个环境和多种定位方法下均被检测到,分别是 Sat_003—Satt306、Satt070
—Satt122 和 Satt571—Satt270。
关键词:大豆;异黄酮;SSR;QTL
Identification of QTLs for Major ponents
Among Multiple Environments in Soybean Seeds
ZHANG Jing-ying, GE Yi-nan, SUN Jun-ming, HAN Fen-xia, YU Fu-kuan, YAN Shu-rong, YANG Hua
(Institute of Crop Science, Chinese Academy of Agricultural Sciences/Ministry of Agriculture (MOA) Key Laboratory of Soybean
Biology, Beijing 100081)
Abstract: 【Objective】The QTLs for major ponents were identified among multiple environments by different
QTL mapping methods in soybean seeds, in order to provide a theoretical basis for soybean isoflavone marker-assisted selection.
【Method】 In this study, a binant inbreed line population (RIL, F5∶7-8) was developed by using the cross between the
isoflavone contrasting cv. LHD2 with high-isoflavone concentration (3 μg·g-1) and cv. NHZ with low-isoflavone
concentration (1 μg·g-1). The polymorphism of SSR markers among the RIL population was analyzed and the major
ponents were determined by HPLC. 【Result】The results showed that a soybean linkage map with a total distance of
3 cM was constructed using 161 polymorphism SSR molecular markers in the RIL population. A total of 14 QTLs associated
with the major ponents were found by ICIM, IM, and SMA methods in the ICIMapping software under the four
environments. 【Conclusion】The three QTLs flanked by the marker interval Sat_003-Satt306,