文档介绍：马钱子碱固体脂质纳米粒的制备研究
吕桦1,宋金春2*,邓睿园1,以盛1
(,湖北武汉 430072;,湖北武汉430060)
摘要:目的:以乳化蒸发-低温固化法制备马钱子碱固体脂质纳米粒并评价其质量。方法:在单因素考察的基础上以正交试验设计优化、筛选最佳处方。用透射电镜观察固体脂质纳米粒的形态, HPLC法测定马钱子碱固体脂质纳米粒的包封率, 激光散射测定Zeta电位和粒度分布,并考察其稳定性。结果:所制固体脂质纳米粒外观形态圆整,平均粒径为116 nm,Zeta电位为-,%。4℃放置1个月,粒径、包封率无明显变化。结论:本研究制备的马钱子碱固体脂质纳米粒粒径分布窄,稳定性好,为开发马钱子碱低毒长效的制剂奠定了实验基础。
关键字:固体脂质纳米粒;马钱子碱;包封率;稳定性;正交试验
[中图分类号] R943; [文献标识码] A
Preparation and Quality Evaluation of Brucine solid lipid nanoparticles
LV Hua1, SONG Jin-chun2*, DENG Rui-yuan1, YI Sheng1 ( of pharmacy, Wuhan university, Wuhan 430072, China ; of pharmacy, Renmin Hospital of Wuhan university, Wuhan 430060,China)
OBJECTIVE:To prepare Brucine solid lipid nanoparticles(Bru-SLN) by means of emulsification- solvent evaporation method and to evaluate its :The formulation of Bru-SLN were optimized by the orthogonal design on the basis of single factor morphology was examined by the transmission electron -performance of liquid chromatography was employed to determine the entrapment efficiency of Bru- diameter,zeta potential and the stability of Bru-SLN were :The obtained solid lipid nanoparticles were round,smooth particles with average size of 116 nm,zeta potential -29 mV and entrapment efficiency %.There were no notable changes of the parti