文档介绍：大白菜黑斑病室内苗期抗性鉴定方法的研究
甘彩霞,祝花,崔磊,袁伟玲,高翔,符义彬,梅时勇
摘要:为了选育大白菜(Brassica campestris L. ssp pekinensis(Lour.) Olsson)抗黑斑病(Alternaria brassicae)新品种和种质创新,在室内利用6个品种的大白菜研究了接种物浓度、苗龄和温度对大白菜黑斑病发病的影响,结果表明,在(25±1)℃光照培养室内,×104个孢子/×105个孢子/mL的黑斑病病菌孢子悬浮液喷雾接种于苗龄为3~4片真叶的大白菜幼苗,保湿24~48 h,10 d后进行病情调查,可对大白菜种质资源抗黑斑病的抗性进行正确评价。
关键词:大白菜(Brassica campestris L. ssp pekinensis (Lour.) Olsson);黑斑病(Alternaria brassicae);苗期;抗性鉴定
Study on Resistance Identification Method for Black Spot of Chinese Cabbage at Seedling Stage in Laboratory
GAN Cai-xia1,ZHU Hua2,CUI Lei1,YUAN Wei-ling1,GAO Xiang1,FU Yi-bin1,MEI Shi-yong1
(1. Institute of Economic Crops, Hubei Academy of Agricultural Sciences,Wuhan 430064,China;
2. Wuhan Vegetable Technical Service Station, Wuhan 430012,China)
Abstract: In order to provide technical support for breeding new varieties and germplasm innovation of Chinese cabbage(Brassica campestris L. ssp pekinensis(Lour.) Olsson) with resistance to black spot(Alternaria brassicae), the influence of inoculat
ion spore concentration, seedling stage and temperature on black spot occurrence was studied with six varieties. It was showed that by spraying ×104 pfu/mL or ×105 pfu/mL spore suspension on seedling at 3~4 leaf stage and then moisturizing for 24~48 h at (25±1)℃ in door, the resistance of radish varieties on the black spot could be accurately evaluated 10 d after ino