文档介绍：三氧化二砷对肝癌HepG2细胞增殖的影响
作者:闻勤生,罗俊卿,焦成松
【关键词】三氧化二砷
关键词: 三氧化二砷;肝癌;砷剂/治疗应用
摘要:目的研究三氧化二砷(As2 O3 )对肝癌细胞的增殖抑制作用. 方法应用台盼蓝拒染法、MTT比色法及细胞克隆形成试验,研究As2 O3 对肝癌HepG2细胞的增殖抑制和细胞毒作用. 结果 As2 O3 能显著抑制肝癌细胞的生长,5μmol・L-1 As2 O3 抑制程度明显强于1μmol・L-1 .5和1μmol・L-1 As2 O3 作用后细胞克隆形成率分别为(±)%和(±)%,明显低于对照组的(±)%(P<).As2 O3 对肝癌细胞有较强的细胞毒作用,且呈明显剂量和时间依赖性,其对肝癌细胞的杀伤率高于5-氟脲嘧啶(5-FU)和丝裂霉素(MMC),但无显著性差异,As2 O3 与5-FU及MMC存在协同效应,联合应用杀伤率明显升高. 结论 As2 O3 具有明显的抑制肝癌细胞增殖作用,有必要进一步探讨其对肝癌的治疗价值.
Keywords:arsenic trioxide;liver neoplasm;arsenicals/TU
Abstract:AIM To explore the inhibitive effect of arsenic trioxide(As2 O3 )on the proliferation of human liver cancer cell line- The morphologic cha
nges,proliferation ability,colony-forming rate of HepG2treated by As2 O3 were studied with light microscopy,growth curve,trypan blue and colony-forming cytotoxicity of As2 O3 on HepG2was determined by MTT Colony-forming rate of HepG2with5μmol・L-1 As2 O3(±)%was lower than that with1μmol・L-1 As2 O3 (±)%,P< much lower than that of con-trol group(±)%,P< O3 had a remark-able cytotoxic effect on HepG2and cytotoxic effect was dose-and-time cytotoxic effect was enhanced through bination of As2 O3 with5-Fu or As2 O3 can significantly inhibit proliferation of HepG2and