文档介绍：缺血再灌注肝一氧化氮合酶活性变化的观察
【关键词】大鼠
摘要:目的探讨大鼠缺血再灌注肝一氧化氮合酶的变化及其意义。方法健康雄性SD大鼠24只,门静脉插管、肝静脉插管、胆道插管,切取肝,建立大鼠离体肝灌注(IPRL)模型。大鼠随机分为4组(每组6只):①热缺血对照组,肝切取后,不作冷保存,立即进行灌注;②冷保存6h组,肝切取后作冷保存,保存6h后进行灌注;③冷保存12h组,肝切取后作冷保存,保存12h后进行灌注;④冷保存24h组,肝切取后作冷保存,保存24h后进行灌注。IPRL采用恒温灌注,、38℃的Krebs-Bülbring buffer液30min。观察胆汁分泌,检测肝静脉流出液中白蛋白(ALB)含量,测定肝组织内皮素-1(ET-1)、一氧化氮(NO)、一氧化氮合酶(NOS)、ATP酶(ATPase)、超氧化物歧化酶(SOD)、黄嘌呤氧化酶(XOD)、丙二醛(MDA)等指标。结果大鼠肝NOS发生了明显的变化,但肝组织NO、ET-1没有显著变化,ATPase、SOD、XOD、MDA的代偿也是有限的。再灌注过程中,各组大鼠肝均有胆汁分泌。肝静脉引流液中ALB含量无差异。结论 NOS的变化是反映缺血再灌注损伤的敏感指标。
关键词:大鼠;肝;缺血再灌注;一氧化氮合酶;酶
Abstract:Objective To study the level of nitric oxide synthase(NOS)in ischemia-reperfusion rat 24healthy male SD rats were anaesthetized and hepatic vein,portal vein and bile ductwere liver was then removed to establish a model of isolated perfusion rat liver(IPRL).The rats were randomly divided into4groups(n=6per group).In GroupⅠ(warm group),the IPRL was formed immediately after livers of GroupsⅡtoⅣwere first stored in cold UW solution for6,12and24hours, livers were then perfused via the portal vein,with freshly prepared Krebs-Bülbring buffer temperature an bath and perfusate was controlled at38℃.Bile secretion was noted;albumin(ALB)was was performed to measure the activities of hepatic en-dothelin-1(ET-1),nitric oxide(NO),nitric oxide synthase(NOS),ATPase,superoxide dismutase(SOD),xanthine oxi-dase(XOD)and malondiaehyde(MDA) The activity of NOSwas increased significantly as the period of cold storage wad prolonged(P<),no distinct changes were found in NO and ET-1,while slight variations were seen in AT-Pase,SOD,XOD and was secreted during the ischemia-reperfusin process in all of the showed no NOS is a sensitive index to reflect the liver injury.
Key words:rat;liver;();nitric oxid