文档介绍：血清中视黄醇结合蛋白免疫透射比浊测定法
王晓蓉
(上海电力医院上海 200052)
摘要:目的对视黄醇结合蛋白免疫透射比浊测定法进行方法学和临床应用评价。方法采用免疫透射比浊测定法,LS相关文件,对方法进行精密度、线性、准确性等评价及临床初步应用。结果批内CV %~% ,批间CV %~% ,日间CV %~% ,总CV %~% ,线性范围0至210 mg/L , r = ,对照组血清视黄醇结合蛋白含量:± mg/L;糖尿病肾病组:± mg/L;慢性肾小球肾炎组:± mg/L;急性肾小球肾炎组:± mg/L;慢性肾功能衰竭:±;肾病组与对照组有显著差异(P〈 )。结论免疫透射比浊法测定血清视黄醇结合蛋白,方法简便、快速、灵敏,结果准确,可用自动分析仪测试,适合临床检验科应用。对肾脏疾病的早期诊断有一定临床意义。
关键词视黄醇结合蛋白免疫透射比浊法肾功能
Turbidimetric Immunoassay for determination of Retinol Conjugated Protein in Serum
Xiaorong Wang . ( Shanghai Power Hospital ,Shanghai, 200052,China)
Abstract:Objective: To evaluate the methodology and clinical application of turbidimetric immunoassay for determination of retinol conjugated protein in serum. Method: According to NCCLS relative documents, evaluate the precision, linearity and accuracy of this turbidimetric immunoassay and try a clinical application. Results: within-lot CV %-%, between-lot CV %-%, between-day CV %-%, total CV %-%, linearity range 0-210mg/L, r = . Quantity of retinol conjugated protein pared Group: ±; Diabetic Renopathy Group: ±; Chronic Glomerulonephritis Group: ±; Acute Glomerulonephritis Group: ± mg/L; Chronic Renal Failure Group: ±. There’s obvious difference between the kidney disease Group and pare