文档介绍：NF-κB在紫杉醇诱发大鼠周围神经疾病中的变化
李君冯艺△王莹张挺杰
【摘要】目的建立紫杉醇诱发大鼠周围神经疾病的模型,研究NF-κB在紫杉醇诱发大鼠周围神经疾病中的变化。方法健康雄性SD大鼠20只,随机分为紫杉醇组(P组)和对照组(C组),每组10例。分别隔日腹腔内注射紫杉醇2mg/kg(P组)或等量生理盐水(C组),共4次。建模前和建模后第7天、第14天和第21天分别测量大鼠的机械痛阈和热痛阈。第21天时免疫荧光法测定L4~6节段脊髓背角NF-κB的表达及星型胶质细胞的数量,Western blot法测量L4~6脊髓背角和背根神经节(DRG)中NF-κB的含量。结果紫杉醇组与对照组相比有体重下降的趋势。D14和D21紫杉醇组的热痛阈显著低于对照组(P<)。紫杉醇组D7、D14、D21的热痛阈依次下降(P<)。紫杉醇组D14和D21 的4g和15g von Frey纤维刺激缩足反应大鼠显著多于对照组(P<)。紫杉醇组大鼠脊髓背角中星型胶质细胞的数量明显低于对照组(P<)。对照组NF-κB主要在大鼠脊髓背角星型胶质细胞的胞浆中表达,紫杉醇组NF-κB主要在大鼠脊髓背角星型胶质细胞的细胞核内表达。L4~6脊髓背角和DRG中NF-κB的含量差异无统计学意义。结论给予紫杉醇后大鼠出现机械痛阈和热痛阈下降、脊髓背角的星型胶质细胞数量减少、NF-κB被激活。提示星型胶质细胞以及NF-κB可能在紫杉醇诱发大鼠周围神经疾病模型中发挥了重要作用。
【关键词】化疗诱发的周围神经疾病;紫杉醇;神经病理性疼痛;NF-κB;星形胶质细胞细胞
Changes of NF-κB in Paclitaxel Induced Peripheral Neuropathy of Rats LI Jun, FENG Yi, WANG Ying, ZHANG Ting Jie. Department of Anesthesiology and Pain Medicine, People’s Hospital of Peking University, Beijing 100044, China
Corresponding author: FENG Yi, Email: yifeng65@
[Abstract] Objective To establish paclitaxel induced peripheral neuropathy model of rats. To investigate changes of NF-κB in paclitaxel induced peripheral neuropathy of rats. Method Twenty healthy SD rats were randomly divided into paclitaxel group(P group) and control group(C group), ten rats of each group. Paclitaxel 2mg/kg ip every other day(P group) and equal volume of saline(C group), 4 times total. Test mechanical pain thresh