文档介绍：丙型肝炎病毒核心蛋白在鼠肝中表达的诱癌作用
作者:韩苏夏,李华,潘承恩,杨广笑,王全颖
【关键词】,丙型肝炎病毒
【Abstract】 AIM: To study the expression in rat liver and carcinogenesis of hepatitis C virus core protein. METHODS: The binant adenoassociated virus (rAAV) containing cDNA of HCV core protein was prepared by using binant virus technology. The livers of SD bandicoots were infected by rAAV. At the 2nd month and 9th month, Southern blot assay and Dot blot assay were used to detect the integrated gene and mRNA of HCV core protein and the titer of the rAAV respectively. Carcinogenesis of hepatitis C virus core protein was tested by HE staining. RESULTS: The titer of the rAAV was ×1014 particles/L. Two and nine months after infection, all the results of Southern blot assay and Dot blot assay were positive. At the 9th month after infection, partial cells of SD bandicoots tended to be malignant. CONCLUSION: The HCV core protein expressed in the liver of SD bandicoots is involved in carcinogenesis.
【Keywords】 hepatitis C virus; viral core proteins; carcinoma, hepatocellular
【摘要】目的: 研究丙型肝炎病毒核心蛋白(HCV core)在鼠肝中的表达和可能潜在的直接致癌作用. 方法: 以病毒重组技术制备含HCV Core区cDNA的重组腺伴随病毒载体. 重组腺伴随病毒感染大鼠肝脏,感染2, 9 mo后分别以Southern blot印迹杂交法和Dot blot杂交法检测鼠肝HCV Core区基因的整合、mRNA形成情况及病毒滴度. HE染色观察诱癌实验结果. 结果: ×1014颗粒数(分子数)/L. 分别感染2, 9 mo后检测大鼠肝脏Southern blot印迹杂交结果和HCV core mRNA均为阳性,HCV core基因为非定点整合. 感染后9 mo实验组大鼠部分细胞有恶性倾向. 结论: HCV core在大鼠肝中持续表达