文档介绍：MPT64 DNA疫苗对鼠结核分枝杆菌感染的保护作用
作者:孙平,骆旭东,朱道银,唐国建
【关键词】结核分枝杆菌
【Abstract】 AIM: To explore the protective efficacy of the DNA ine encoding Mycobacterium tuberculosis MPT64 in mice infected with Mtb. METHODS: C57BL/6 mice were intramuscularly immunized with the DNA ines or BCG (.). The mice were challenged with 106 CFU H37Rv via lateral tail vein 35 d after the third immunization for DNA ine groups and 100 d after for BCG inated group. The mice in the inated groups and control groups were sacrificed 42 d after challenge. The lungs and spleens were removed respectively and the number of CFU ans and histopathologic changes were determined. The antibody level, IFNγ, IL4 and the survival time in all of the mice were evaluated. RESULTS: The antibody titer of pcDNA/MPT64 group was higher than that of other groups (P<). Both the level of IFNγ produced by spleen lymphocytes and the spleen lymphocyte proliferation from BCG group and pcDNA/MPT64 group were higher than those of other groups (P<), and no IL4 was found in all groups. The number of bacterial colonies in the lungs and spleens significantly decreased at 6th week postchallenge in all the inated groups (P<), especially in BCG group (P<). The pulmonary histopathological changes were observed 6 weeks following challenge with H37Rv. In PBS and groups, the lesion was characterized