文档介绍：唐古特大黄多糖及其组分的抗氧化损伤作用
作者:刘莉梅其炳李保莉周四元曹之宪
【关键词】大黄
关键词: 大黄;多糖类;肝/损伤;丙二醛;谷胱甘肽过氧化酶
摘要:目的比较唐古特大黄多糖(TMP)及其组分TMP-I对小鼠急性肝损伤的保护作用,并探讨其作用机制. 方法保护作用:小鼠190只,随机分19组,预先以TMP100,200和400mg・kg-1 ,TMP-I50,100mg・kg-1 灌胃(ig)给药,2次・d-1 ,5d;l4 (156mg・kg-1 )、D-Gal-N(800mg・kg-1 )和TAA(550mg・kg-1 )腹腔注射(ip)复制小鼠急性肝损伤模型,并用生理盐水做对照;应用硫代巴比妥酸比色法测定肝组织MDA含量,:小鼠90只,随机分9组,以TMP200mg・kg-1 ,400mg・kg
-1 ,TMP-I50mg・kg-1 ,100mg・kg-1 ig,ip给药,2次・d-1 ,5d;应用硫代巴比妥酸比色法测定肝组织MDA含量,DTNB直接法测定肝组织和血清中GSH-PX的活性. 结果唐古特大黄多糖能显著提高TAA引起肝损伤动物存活率,降低各模型组动物肝组织MDA含量,降低血清中ALT,AST水平,两种给药途径ig,ip均能使正常小鼠肝组织GSH-PX活性增加. 结论 l4 ,D-Gal-N和TAA引起的小鼠急性肝损伤具有明显的保护作用,TMP-I可能是其有效组分,唐古特大黄多糖抗肝损伤的作用可能与其抗氧化作用有关.
Keywords:Pheum officinale;harides;liver/injury;malondialdehyde;glutathione peroxidase
Abstract:AIM To investigate the protective effects of Tan-guficum Maxim haride(TMP)and its fractions(TMP-I)on liver injury in Three acute liver injury models of mice were made l4 (156mg・kg-1 ),D-Gal-N(800mg・kg-1 ),TAA(550mg・kg-1 )ip respectively,following the administration of TMP100,200and400mg・kg-1 ,TMP-I50mg・kg-1 and100mg・kg-1 TMP and its fraction TMP-I in-creased the rate of survival in TAA model and decreased sig-nificantly MDA level in liver and activity of ALT and AST in serum in the three administration both ig and ip showed evident effects in reducing MDA level and increasing GSH-PX Liver protective actions of the haride extracted from Tanguficum Maxim may relate to its antioxidation.
0 引言
唐古特大黄系蓼科植物rheum tanguficam max-im ex balf L的干燥根及根茎,、破积滞、行瘀血、攻瘀滞、泄湿热、退黄疸等作用,广泛用于痢疾、肝炎,[1] ,为进一步探讨大黄利胆、肝损伤防护作用的药理学基础,我们将大黄总多糖再度分离纯化得到大黄多糖-I,采用小鼠急性肝损伤模型进行观察比较,以期探讨其急性肝损伤的保护作用及其机制.
1 材料和方法
材料
唐古特大黄总多糖(TMP)的制备购自青海省医药公司的唐古特大黄经乙醇、沸水回流,浓缩,除蛋白,醇沉,冷冻干燥,-I的制备:取500m