文档介绍：陕西汉族人群ER基因扩增片段酶切长度多态性
作者:李军林, 舒青,郑强荪,冯蕾
【关键词】,雌激素受体基因
【Abstract 】 AIM: To study the distribution of ER intron 1 polymorphism in Han population of different ages and sexes in Shaanxi Province. METHODS: AmplificationRestriction Fragment length Polymorphism (AmpRFLP) was used to analyze the polymorphism of Han population in Shaanxi Province. The templates of PCR were extracted from 162 independent cases of different ages and sexes in Shaanxi Province. RESULTS: ① The full length of ER intron I after PCR was kb. ② Using restriction endonuclease enzymes, three types of genotypes were obtained: Pvu II: P/P ( kb), P/p ( kb, 850 and 450 bp) and p/p (850 bp, 450 bp). ③ Using restriction endonuclease enzymes, another three types of genotypes were obtained: Xba I : X/X ( kb), X/x ( kb, 910 and 390 bp)and x/x(910 bp, 390 bp). CONCLUSION: It is suggested that ER gene intron 1 of Han population in Shaanxi Province is polymorphism. The distribution of the genotype is not related to different age groups and different gender groups.
【Keywords】 ER gene;gene polymorphism;Han population in Shaanxi province
【摘要】目的: 分析陕西省不同年龄和不同性别的汉族人群雌激素受体(ER)基因1号内含子基因多态性分布情况. 方法: 研究基于PCR后Pvu II和Xba I的酶切结果,PCR模板DNA来自陕西省162例无关个体,研究对象分为不同年龄和不同性别两组. 结果: ① kb;②经PvuⅡ酶切,得到3种ER基因型:P/P型( kb),P/p型( kb
,850和450 bp),p/p型(850和450 bp);③经Xba I酶切得到的3种1号内含子基因型为:X/X型( kb),X