文档介绍：异氟醚预处理脑保护作用的剂量-效应关系
作者:吴明春熊利泽朱正华侯立朝郑玉桑韩飞
【关键词】异氟醚
关键词: 异氟醚;预处理;脑缺血;剂量效应
摘要:目的研究异氟醚预处理脑保护作用的剂量效应关系. 方法 40只雄性SD大鼠,随机分为4组:对照组,Iso1,Iso2和Iso3组(n=10).对照组大鼠每日吸4L min-1 (970mL L-1 O2 )O2 1h,,, L-1 的异氟醚预处理(异氟醚和4L min-1 O2 ),每日1h,,用右侧颈内动脉尼龙线线栓法制作大脑中动脉阻闭(mid-dle cerebral artery occlusion,MCAO). 结果 Iso2和Iso3组大鼠神经功能障碍及脑梗死容积均与对照组及Iso1组相比差异显著(P<).Iso3组大鼠神经功能障碍及脑梗死容积与Iso2组相比差异显著(P<). 结论异氟醚预处理需达到一定浓度才能诱导脑缺血耐受,并存在一定的剂量效应关系.
Keywords:isoflurane;preconditioning;cerebral ischemia;dose-response
Abstract:AIM To study the dose-response effects of isoflu-rane preconditioning to induce ischemic tolerance in transient middle cerebral artery occlusion model in Forty male SD rats were randomly assigned to4groups:the control group,Iso1group,Iso2group and Iso3group,with10in each animals in the control group received4L min-1 O2 1h everyday animals in the last three groups , L-1 isoflurane in4L min-1 O2 1h everyday for5d after the last treatment,transient MCAO was induced by introduc-ing a3-nylon monofilament suture through right internal carotid deficit scores and infarct volume were used to assess the effects of isoflurane The neurologic deficit scores and infarct v