文档介绍：2-(2-苯并呋喃基)-2-咪唑啉对星形胶质细胞糖氧剥夺损伤的保护及抗脂质过氧化作用
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作者:田继沙,韩钊,张红霞,肖美娟,杨金龙,程朝辉,王小同
【摘要】目的:探讨2-(2-苯并呋喃基)-2-咪唑啉(2-BFI)对星形胶质细胞糖氧剥夺(oxygen-glucosedeprivation,OGD)损伤的保护作用及脂质过氧化的影响。方法:制成星形胶质细胞糖氧剥夺损伤模型,分为正常对照组、OGD组、OGD+2-BFI干预组(、、3、6和12μg/mL),测定星形胶质细胞存活率,选出抗OGD损伤最佳浓度,以该浓度进一步观察2-BFI对脂质过氧化产物丙二醛(MDA)和还原型谷胱甘肽(GSH)含量的影响。结果:星形胶质细胞在OGD损伤后,不同浓度的2-BFI均能提高细胞存活率,; 2-BFI干预OGD组能显著降低MDA含量,增加GSH含量。结论:2-BFI能保护星形胶质细胞OGD损伤,且有抑制脂质过氧化的作用。
【关键词】咪唑啉受体;2-BFI;糖氧剥夺;脂质过氧化;大鼠
Abstract: Objective: To explore the neuroprotective effects and lipid peroxidation of 2-(2-benzonfuranyl)-2-imidazoline on oxygen-glucose deprivation of cultured rat corticalastrocytes. Methods: The model of oxygen-glucose deprivation of cultured rat cortical astrocyteswas were designed into three groups: normal control group,oxygen-glucosedeprivation(OGD) group and oxygen-glucose deprivation+2-BFI intervention group (the concentration of drug was ,,3,6 and 12μg/mL),to determine the cell survival rate of astrocytes,the best drug concentration was chosen,then the best drug density was used to measure the content of malondialdehyde(MDA) and reduced glutathione (GSH). Results: When astrocytes were deprived of oxygen and glucose,different concentration of 2-BFI couldsignificantly raised the cell survival rate,and the effect of was the best; g/mL 2-BFI intervention group could significantly reduced MDA level,and raised the content of reduced glutathione. Conclusion: 2-BFI can effectively protect astrocyte injury afteroxygen-glucose deprivation,and inhibit lipid peroxidation.
Key words: imidazoline receptors;2-BFI;oxygen-glucose deprivation;lipid peroxidation;rats
1984年Bousquet首次提出咪唑啉受体(imida-zoline receptors,IR)概念,迄今的研究认为该受体有两种亚型,一种是与可乐定有高亲和力的咪唑啉I1受体(I1R),主要存在于延髓头端腹外侧,与血压的调节有关;另一种是与咪唑克生有高亲和力的咪唑啉I2受体(I2R),在人体广泛存在于神经系统、心血管系统、肾脏等组织。2-(2-苯并呋喃基)-2-咪唑啉〔2-(2-benzofu-ranyl)-2-imidazoline, 2-BFI〕是近年来才新发现的高亲和力I2R配体。我们的前期研究发