文档介绍：APC基因异常甲基化在上皮性卵巢癌中的检测及临床意义
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作者:孙健卢健邹冬芳吴
【摘要】目的观察结肠腺瘤性息肉病(APC) 基因异常甲基化在上皮性卵巢癌中的作用及临床意义。方法 59例上皮性卵巢癌组织原发灶,32例相应的盆、腹腔转移灶,12例癌旁卵巢组织及30例正常卵巢组织,采用甲基化特异性聚合酶链式反应(MSP)法检测APC基因启动子区甲基化状态。结果上皮性卵巢癌组织原发灶及相应的盆、腹腔转移灶中存在APC基因启动子区异常甲基化,%、%,显著高于正常卵巢组织()。12例癌旁卵巢组织中1例检测到APC基因启动子区甲基化,30例正常卵巢组织未检测到。APC基因启动子区甲基化的发生率在临床Ⅰ、Ⅱ期低于Ⅲ、Ⅳ期(),在高分化癌中低于低分化癌()。结论 APC基因启动子区异常甲基化与上皮性卵巢癌发生密切相关,并可能与上皮性卵巢癌临床分期及分化程度有关。
【关键词】卵巢癌; APC基因; DNA甲基化
【Abstract】 Objective To observe the role of promoter hypermethylation of adenomatous polyposis coli (APC) gene in the course of epithelial ovarian carcinoma. Methods Hypermethylation of APC promoter was detected by methylation specific PCP (MSP) in 59 epithelia
l ovarian cancer primary tissues, 32 metastatic tissues of pelvic and abdomen cavity, 12 adjacent noncancerous ovarian tissues and 30 normal ovarian tissues. Results Hypermethylation of APC promoter was detected in ovarian cancer tissues and metastatic sites,the frequency being % and % respectively, which was significantly higher than that in normal ovarian tissues().Hypermethylation of APC promoter was detected in 1 out of 12 adjacent noncancerous ovarian tissues,and no hypermethylation was detected in normal ovarian frequency of hypermethylation of APC promoter was significantly lower in cancers of stage I and Ⅱ th