文档介绍：CD41标记的微小巨核细胞染色在老年贫血诊断中的应用
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作者:邢颖,曲妍,黄佳玲,林树东
【摘要】目的:探讨CD41标记的微小巨核细胞染色法(SAP染色法)在老年贫血诊断及鉴别诊断中的意义。方法:采用瑞特姬姆萨染色和CD41标记的SAP染色法,分别检测了107例60岁以上老年贫血患者的骨髓涂片中微小巨核细胞的数量和类型。结果:CD41标记的SAP染色法对巨核细胞的检出率明显高于瑞特姬姆萨染色法,两者相比差异有统计学意义()。不同疾病组比较,微小巨核细胞阳性率MDS组最高,依次为MDSITPMAACDMMIDARAAAPRCACAA;%,其他组均未检出。结论:CD41标记的SAP染色可以显著提高巨核细胞检出率;且对老年贫血具有一定的鉴别诊断价值。
【关键词】 CD41单抗; 微小巨核细胞; SAP染色; 老年贫血; 鉴别诊断
[Abstract] Objective: To assess the significance of the CD41 labelled micromegakaryocyte StreptavidinAlkaline Phosphatase(SAP) staining in the diagnosis and differential diagnosis of senile anemia. Methods: Bone marrow smears from 107 patients with anemia over the age of 60 were detected by Wright
Giemsa staining and CD41 labelled SAP staining for the number and types of micromegakaryocytes, respectively. Results: The detection rates of megakaryocyte by the CD41 labelled SAP staining and WrightGiemsa staining had significant difference(). Among the groups, MDS group for the positive rate of micromegakaryocyte ranked the first, followed by ITPMAACDMMIDARAAAPRCACAA; Detection rate of lymphoid micromegakaryocyte in the MDS group was %, there were not micromegakaryocytes observed in other groups. Conclusion: CD41 labelled SAP staining obviously increases the detection rate of megakaryocyte, which is valuable for differential diag