文档介绍：c-myc、c-fos在良性胆道狭窄瘢痕组织中的表达及意义
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【摘要】目的探讨原癌基因c-myc、c-fos与良性胆道狭窄形成的相关性。进一步阐明良性胆道狭窄中胆管瘢痕的发生机制。方法应用免疫组化SP法,检测c-myc、c-fos蛋白在肝内、外胆管瘢痕组织中的表达和分布。对其阳性表达结果进行比较分析。结果肝内、外胆管瘢痕组织的成纤维细胞、胆管上皮细胞及血管内皮细胞中c-myc、c-fos呈强阳性表达,而正常胆管组织中缺乏表达。结论肝内、外胆管瘢痕组织中c-myc和c-fos癌基因受激活,可能参与了成纤维细胞的分化增殖、胶原合成与降解以及对细胞因子的调控,并导致瘢痕增生。原癌基因c-myc、c-fos与良性胆道狭窄瘢痕形成密切相关。c-myc与c-fos蛋白两者的表达成正相关,两者具有协同性。
【关键词】原癌基因c-myc 原癌基因c-fos 良性胆管狭窄胆管瘢痕
Expression of c-myc、c-fos in scartissue of benign bi
liary stricture and correlation investigation
LU Hui,ZHANG Xiao-wen,LIU Wen- of Surgery,Fengcheng Hospital,Shanghai 201411,China
【Abstract】 Objective To observe the expression and distribution of c-myc and c-fos in biliary scar tissue, to investigate the correlation between expression of key pro-oncogenes and benign biliary stricture. Methods Immunohistochemical technique was used to detect the expression and distribution of c-myc、c-fos proteins on extrahepatic bile duct scar tissue、intrahepatic bile duct scar tissue and normal bile duct tissue. Results c-myc and c-fos expression on the nucleus of fibroblast、epithelial cell and endothelial cell of biliary scar tissues were significantly higher than normal biliary tissue. And there was obviously difference between scar tissue and normal c-myc and c-fos oncogenes are activated on biliary scar tissues,which m