文档介绍：BALB/c小鼠黑色素瘤B16移植肿瘤模型的建立
(作者:___________单位: ___________邮编: ___________)
【摘要】[目的]建立宜于进行早期观察及测量的小鼠黑色素瘤模型[方法]将不同数量的处于对数生长期的B16细胞株黑色素瘤细胞接种于BALB/c小鼠背部及足垫皮下,,应用病理学和免疫组织化学染色方法进行鉴定,归纳总结接种细胞数与肿瘤发生率的关系及肿瘤生长对小鼠生活状态的影响[结果]接种5d后BALB/c小鼠皮肤浅表部位相继出现黑色的点或线状改变,发展为结节状改变直至肿瘤破溃、出血、死亡或破溃、出血、结痂、愈合,×105个时BALB/c小鼠的肿瘤模型全部建立成功[结论]B16细胞株黑色素瘤细胞接种于BALB/c小鼠的肿瘤模型成功率与接种细胞数成正比,它具有建立肿瘤模型成功率高,观察及测量方便等优点,接种部位以背部皮下为宜.
【关键词】黑色素瘤模型动物小鼠
ABSTRACT:OBJECTIVETo establish neoplasm transplantation models of melanoma in BALB/c miceMETHODSB16 melanoma cells in logarithmic growth phase were inoculated into BALB/c mice’s back hypodermis and feet with different concentration to establish neoplasm transplantation of melanoma. The growth characteristics of transplanted melanoma in mice were observed and the tumor properties were identified by histopathology and immunohistochemistry. The relations between the number of B16 cells inoculated and ic rate, and the influence of tumorigenesis for mice were observedRESULTSFive days later, black spots and linear changes were gradually seen in BALB/c mice’s back skin, feet or ankle, and then they developed to tubercles, ulceration, hemorrhage and death. The ic rate in inoculating with 5×105 B16 cells was near 100%CONCLUSIONThe ic rate has a positive relation to the number of inoculated B16 cells, and is high in BALB/c mice inoculated the B16