H22小鼠肝癌细胞接种数与实验性肺转移的相关性.doc

文档介绍：H22小鼠肝癌细胞接种数与实验性肺转移的相关性
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【摘要】目的:探讨H22小鼠肝癌细胞接种数与实验性肺转移的相关性。方法:昆明种小鼠尾静脉注射不同数量(1×107、×106、5×106、×106、1×106)H22细胞制作实验性肺转移模型,肉眼及镜下观察肺内肿瘤的有无、大小、转移程度及分布情况,血清ICAM1、IL2蛋白的表达情况。结果:接种1×107细胞浓度组小鼠肺转移率、恶化程度及血清中ICAM1蛋白表达的量均高于其他接种组(),IL2蛋白表达低于其他接种组(),其中部分小鼠有脑转移。结论:小鼠实验性肺转移的发生率、转移程度及血清中ICAM1、IL2蛋白的表达量与细胞接种数变化密切相关。
【关键词】肝癌;ICAM1;IL2;实验性肺转移
Abstract:[Objective]To study correlation cell number of mouse H22 liver carcinoma with occurrence of the experimental pulmonary metastasis.[Method]The Kunming mouse experimental pulmonary metastatic model of different H22 liver carcinoma cell numbers was established by injecting tail vein;distribution,size and grade of metastatic nodes were observed
by naked eye and microscope,expressing of ICAM1 and IL2 in blood serum of mouse was detected.[Result]Rate and grade of pulmonary metastasis and expressing of ICAM1 in blood serum were higher for mouse of injecting1×107 cell number than other groups,and lower for expressing of IL2 in blood serum obviously,some had brain metastases among these mice.[Conclusion]The rate and grade of mice experimental pulmonary metastasis and expressing of ICAM1,IL2 are correlated with cell number.
Key words: hepatoma;ICAM1;IL2;experimental pulmonary metastasis
大量证据表明原发肿瘤早期阶段有恶性肿瘤细胞脱落到循环系统[1],这些脱落的恶性肿瘤细胞可导致肿瘤复发和转移,外周血循环肿瘤细胞是一个恶性肿瘤扩散的表现,对这些肿瘤细胞的检测