文档介绍：hVEGF165在兔骨髓间充质干细胞中的表达
(作者:___________单位: ___________邮编: ___________)
作者:王宾沈来根朱越锋李鲁滨郭治宇刘振杰
【摘要】目的培养和鉴定兔骨髓间充质干细胞,基因转染后观察hVEGF165在其中的表达。方法体外培养兔骨髓间充质干细胞,以流式细胞仪检测其表面抗原(CD29、CD44、CD45、HLA-DR)的表达。通过腺病毒载体转染hVEGF165后,采用RT-PCR、Western blot法检测hVEGF165在细胞中的表达。结果通过流式细胞仪检测表明培养的细胞为兔骨髓间充质干细胞, RT-PCR法、Western blot法检测结果均表明hVEGF165基因转染兔骨髓间充质干细胞后能在其中表达。结论成功培养兔体外骨髓间充质干细胞, 基因转染后细胞能有效表达hVEGF165。
【关键词】目的培养和鉴定兔骨髓间充质干细胞,基因转染后观察hVEGF165在其中的表达。方法体外培养兔骨髓间充质干细胞,以流式细胞仪检测其表面抗原(CD29、CD44、CD45、HLA-DR)的表达。通过腺病毒载体转染hVEGF165后,采用RT-PCR、Western blot法检测hVEGF165在细胞中的表达。结果通过流式细胞仪检测表明培养的细胞为兔骨髓间充质干细胞, RT-PCR法、Western blot法检测结果均表明hVEGF165基因转染兔骨髓间充质干细胞后能在其中表达。结论成功培养兔体外骨髓间充质干细胞, 基因转染后细胞能有效表达hVEGF165。
【Abstract】 Objective To cultivate and accredit rabbit mesenchymal stem cells ,and to detect the expression of hVEGF165 after gene transfection. Methods Rabbit mesenchymal stem cells was accredited expression of antigen CD29、CD44、CD45、HLA-DR after cultivated in vitro. The constructed plasmids containing right sequence of hVEGF165 were transfected into rabbit mesenchymal stem cells by expression of hVEGF165 was tested by RT-PCR and western-blot. Results The expression of antigen of cells indicated the cells were rabbit mesenchymal stem cells. The results of RT-PCR and western-blot showed that the transfected cells could express hVEGF165. Conclusion The rabbit mesenchymal stem cells can essfully express hVE