文档介绍：IL23/IL17轴在反流性食管炎大鼠中的表达及其意义
(作者:___________单位: ___________邮编: ___________)
作者:徐中,赵健雄,王学习,祁永福,许馨文
【摘要】目的检测反流性食管炎大鼠食管黏膜IL23p19、IL17表达水平的变化,探讨IL23/IL17轴在反流性食管炎发病中的作用。方法将30只SD大鼠随机分为3组:空白组、假手术组、模型组。通过半幽门结扎联合贲门肌切开术制备反流性食管炎模型,模型制备后,各组动物继续禁食24h(但不禁水),术后24h给予进食,继续喂养4周后处死大鼠,HE染色法观察病理变化;实时荧光定量RTPCR检测食管组织IL23p19和IL17的mRNA表达;ELISA法检测食管组织IL17蛋白的含量。结果 4周后,与正常组比较,模型组大鼠食管黏膜组织有不同程度的炎症表现。模型组食管组织IL23p19、IL17mRNA表达水平和IL17蛋白的含量明显高于正常对照组、假手术组,后两者之间无统计学差异。结论 IL23/IL17轴是参与反流性食管炎发病的重要的细胞因子,可能参与了反流性食管炎的慢性炎症过程。
【关键词】反流性食管炎;IL23;IL17
ABSTRACT: Objective To detect the expressions of interleukin
17 (IL17) and interleukin23 (IL23) in esophageal tissues of rats with reflux esophagitis (RE) and explore the role of IL23/IL17 axis in the pathogenesis of Thirty SD rats were randomly divided into 3 groups, namely, control group, shamoperated group and model group. RE was induced by adopting partial pyloric ligation plus cardiomyotomy. Then rats in each group were fasted for 24h but had free access to water. They were fed 24h after operation. Four weeks later, rats were killed and pathological changes of esophagus mucous membrane were observed by HE staining in each group. The expressions of IL23p19 and IL17 mRNA in esophageal tissues were examined by realtime fluorescent quantitative reverse transcriptase polymerase chain reaction (RTFQPCR). IL17 protein content in esophageal tissues was measured by After four weeks, compared with control group, model group had significant pathological changes of esophagus mucous membrane. Expressions of IL23p19 and IL17 mRNA in the esophageal tissues of model group were significantly higher than those of the other two groups; the content of IL17 protein was significant higher in mode
l group than in the other two groups. There were no significant differences between control group and shamoperated IL23/IL17 axis is an important cellular factor involved in the pathogenesis of RE and ma