文档介绍：癌基因C-K-ras和抑癌基因Rb突变与胰腺癌发生的关系
(作者:___________单位: ___________邮编: ___________)
作者:关泽红刘旭东刘淑萍云志厚孟化张建宇姜彩虹
【摘要】目的:探讨癌基因C-K-ras和抑癌基因Rb点突变与胰腺癌发生的关系。方法:选择经病理学证实的原发性胰腺癌19例、胰腺癌恶性淋巴瘤1例、胰腺多形细胞癌2例和慢性胰腺炎5例的石蜡包埋组织样本,采用PCR-SSP法检测C-K-ras 12位密码子和Rb第21位外显子的点突变情况。结果:19例原发性胰腺癌石蜡包埋块样本中有7例C-K-ras 12密码子发生了点突变,%,其中1例为两种突变形式(GAT、GTT);有9例Rb第21位外显子发生了点突变,%;而在胰腺癌恶性淋巴瘤、胰腺多形细胞癌和慢性胰腺炎样本中均未检出上述突变情况。结论:C-K-ras 12位密码子和Rb第21外显子点突变与胰腺癌的发生有一定的相关性。
【关键词】胰腺癌;癌基因C-K-ras;抑癌基因Rb;点突变

Abstract Objectives: To study the correspondence of point mutation of oncogene C-K-ras and antioncogene Rbwith the occurrence of pancreatic carcinomas. Methods: The point mutation of C-K-ras at 12 codon and Rb at 21thexon were detected by PCR-SSP in the araffin embedding samples of 19 cases of pancreatic carcinomas ,1 case ofpancreatic lymphoma , 2 cases of pancreatic polymorphous cell carcinomas and 5 cases of cronic : C-K-ras mutation at 12 codon were detected in 7 cases of the 19 pancreatic carcinoma samples (37%), oneof which displayed two kinds of mutation (GAT and GTT); Of 19 cases of pancreatic carcinomas,9 cases were detectedwith mutation of Rb 21th exon (%); No mutation in C-K-ras 12th codon and Rb 12 exon were detected in cronicpancreatitis,pancreatic lymphoma and pancreatic polymorphous cell carcinomas samples. Conclusion: There is somecorrespondence of point mutation of oncogene C-K-ras a