文档介绍：SV40大T抗原在人脑肿瘤中的表达
作者:甄海宁章翔张志文黄文晋张萍梁景文刘先珍
【关键词】 SV40
关键词: SV40;大T抗原(Tag);脑肿瘤;p53;pRb
摘要:目的探讨SV40早期区域基因编码产物大T抗原(Tag)的表达及与抑癌蛋白p53,pRb的相互作用在人脑肿瘤发生发展中的意义. 方法采用免疫共沉淀结合银染色及Western印迹法检测65例人脑肿瘤及8例正常人脑组织中Tag的表达,并对18例和15例Tag阳性瘤组织分别检测Tag-p53和Tag-pRb复合物的形成. 结果 SV40Tag在人脑肿瘤中广泛表达,阳性率为66%(43/65),而8例正常人脑组织均无Tag表达,二者差异显著(P<);分别检测18例和15例Tag阳性瘤组织,均发现Tag可与p53,pRb形成特异性复合物. 结论 SV40Tag的表达与人脑肿瘤的发生有一定关系;SV40Tag与p53,pRb形成特异性复合物并导致其失活,可能是SV40致人脑肿瘤发生的一个重要机制.
Keywords:simian virus40(SV40);large tumor antigen(Tag);brain neoplasms;p53;pRb
Abstract:AIM To study the expression of SV40early re-gion gene coding product,the large tumor antigen(Tag),and its interaction with tumor suppressors p53and pRb in
human brain Tag was investi-gated by immunoprecipitation followed by silver staining and Western blot in65cases of human brain tumors and8cases of normal brain -p53and Tag-plexes were screened by immunoprecipitation and Western blot in18and15Tag positive tumor tissues SV40Tag was expressed generally in human brain tumors with a positive rate66%(43/65),while in8normal brain tissues Tag was negative in all was a significant difference between them(P<).Tag-plex was detected in all of18Tag positive tumors,and Tag--plex was positive in all of the15Tag positive ┐CLUSION SV40Tag expression is associated with h