文档介绍：不同频率电磁场对胆囊癌细胞增殖及DNA损伤作用
作者:陈平,杨耀琴, 陶惠红,杨虎川
【关键词】电磁场
摘要:目的探讨不同频率的低功率电磁场对胆囊癌细胞恶性增殖及DNA损伤的作用及其意义。方法采用绘制细胞生长曲线及单细胞凝胶电泳技术分别检测竟不同频率电磁场作用后,胆囊癌细胞的增殖及DNA损伤情况。结果经不同频率(~40 MHZ)电磁场作用后,各实验组总体上均可见细胞增殖受到抑制,DNA链产生断裂损伤,统计学检验较正常对照有显著意义。~40MHZ范围低功率电磁场对胆囊癌细胞增殖及DNA损伤产生明显影响,主要表现为使DNA链产生断裂损伤,进一步抑制细胞增殖,且这种作用和频率有关,但并不是“线性”关系。
关键词: 频率;电磁场;单细胞凝胶电泳;细胞培养;胆囊肿瘤; DNA损伤
Abstract: Objective To study the effects of low-power ic fields of different frequencies on proliferation and DNA damage of gallbladder cancer cells. Methods Cell growth curve was drawn and single cell gel electrophoresis performed to evaluate the proliferation and DNA damage of gallbladder cancer cells respectively after the cells were exposed to ic fields of different frequencies. Results After exposure to low-power ic fields of different frequencies (-40 MHz), the cells displayed significant changes with obvious cell proliferation inhibition and DNA strand breakage. Conclusion Low-power ic fields within the range of -40 MHz may impair the DNA strand and cause inhibition of proliferation of the gallbladder cancer cells, and these effects are related to the frequency of the ic fields but not in a linear fashion.
Key words: frequency, ic field; single cell gel electrophoresis; cell culture; gallbladder cancer; DNA damage
电磁场可分为长波、中波、短波、射频、微波、极低频率电磁场。在一定范围内,微波对较小极性分子作用明显,而波长较长的射频电磁场则对极性大分子有较强影响。电磁场的生物学作用,由于其生物物