文档介绍：鳖甲炮制前后抗肝纤维化有效物质部位HPCE指纹图谱的比较研究
作者:施婧妮,陈进文,高建蓉,唐尹萍,胡春玲,周莉,刘焱文
【摘要】目的建立生鳖甲与醋鳖甲抗肝纤维化有效物质部位的高效毛细管电泳(HPCE)指纹图谱,探讨其炮制前后药效物质的差异。方法运用毛细管区带电泳法,75 μm×57 cm 未涂层石英毛细管,运行缓冲液为20 mmol/L磷酸氢二钠-100 mmol/L三羟甲基氨基甲烷(l mol/L ),分离电压为18 kV,检测波长为254 nm,柱温为20 ℃,分别建立了10批生鳖甲和10批醋鳖甲有效物质部位的指纹图谱,并进行对比研究。结果生鳖甲指纹图谱共标定了17个共有峰,;醋鳖甲指纹图谱共标定了19个共有峰,;而10批生鳖甲图谱与醋鳖甲对照指纹图谱的相似度降低,~;且10批醋鳖甲图谱与生鳖甲对照指纹图谱的相似度亦降低,~。结论鳖甲炮制前后化学成分及其含量发生了变化,且炮制后产生了一些新的有效成分。
【关键词】鳖甲;炮制;有效成分;高效毛细管电泳;抗肝纤维化;指纹图谱
Abstract:Objective A fingerprint method was established paring the same geographical origin of pre-and-post-processed Trionyx sinensis carapace in anti-liver fibrosis, 10 crude samples and 10 vinegar- processed were anal
yzed. Method Using HPCE detection method equipped with uncoated fused silica capillary column (75 μm×57 cm), with 20 mmol/L Na2HPO4-100 mmol/L Tris (pH=12) as background electrolyte, UV detector at 254 nm, voltage of 18 kV and detection temperature of 20 ℃. Results Seventeen co-possessing peaks were selected as the fingerprints of 10 crude samples, the similarity of the fingerprint were above . een co-possessing peaks were selected as the fingerprints of 10 vinegar-processed samples, the similarity of the fingerprint were above . The similarity between 10 crude samples and the contrast fingerprint of vinegar-processed samples dropped to ~,