He┐Ne激光照射对大鼠神经损伤后脊髓CGRP表达的影响.doc

文档介绍：He┐Ne激光照射对大鼠神经损伤后脊髓CGRP表达的影响
作者:王冰水易南李玲马虹王建波王虹
【关键词】激光
关键词: 激光;大鼠;脊髓;降钙素基因相关肽
摘要:目的研究10mW氦氖(He-Ne)激光照射对大鼠坐骨损伤神经后脊髓CGRP表达的影响. 方法 96只SD大鼠制成坐骨神经损伤模型,He-Ne激光照射损伤的坐骨神经,免疫组化方法观察脊髓内CGRP的变化. 结果神经损伤后1d脊髓内CGRP表达即增加;2wk及4wk时激光照射组CGRP的表达高于对照组,阳性表达主要存在于脊髓灰质内前角运动神经元和背根感觉纤维;8wk时CGRP仍保持较高水平的表达,二组无明显差异. 结论 10mW He-Ne激光照射大鼠损伤的坐骨神经可引起脊髓内神经元CGRP表达的变化,有促进完成神经再生的作用.

Keywords:laser;rat;spinal cord;calcitonin gene-related peptide

Abstract:AIM To study the expression of calcitonin gene-related peptide(CGRP)in spinal cord after irradiation on in-jured sciatic nerve of rat by He-Ne laser with output power Injured sciatic nerves,made in96SD rats,were irradiated by He-Ne changes of CGRP in spinal
cord were immunohistochemically ┐SULTS Expression of CGRP increased in spinal cord on the first day after injury of sciatic expression of CGRP in second and fourth week was observed in laser irradi-ation pared to control positive sub-stance was mainly found in motor neuron in anterior horn and sensory nerve fiber in posterior eighth week,CGRP remained a high expression in both groups,and showed no significant Expression of CGRP in spinal cord can be changed by He-Ne laser irradiation with power output of10mW on injured sciatic nerve in -Ne laser irradiation can promote nerve regeneration.

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